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Invitrogen™ ViraPower™ UbC Lentiviral Gateway™ Expression Kit

Product Code. 17115999
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Quantity:
1 kit
Unit Size:
Each
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Regulatory: Restrictions may apply to the purchase of this product and we may need to request additional documentation from you

Product Code. 17115999

Brand: Invitrogen™ K499000

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This item is not returnable. View return policy
Regulatory: Restrictions may apply to the purchase of this product and we may need to request additional documentation from you

Description

Includes

pLenti6/UbC/V5-DEST (Box 1): 6μg; pLenti6/UbC/V5-GW/lacZ Control vector (Box 1): 10μg; Stbl3 Chemically Competent E. coli (Box 2): 21 x 50μL; pUC19 control DNA (10pg/μL): 50μL; S.O.C. Medium: 6mL; Support kit (Box 3); ViraPower Packaging Mix (1μg/μL): 195μg; Lipofectamine 2000: 0.75mL; Blasticidin: 50mg; 293 FT cells (3 x 106/mL) (Box 4): 1mL

Facilitates easy recombination-based cloning and lentiviral-based expression of a target gene in dividing and nondividing mammalian cells

  • Contains all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit
  • The pLenti6/UbC/V5-DEST vector has the UbC promoter for driving constitutive but physiological levels of expression of the target gene and the blasticidin selection marker for stable selection in mammalian cells
  • Well-suited to long-term experiments
  • Accurate titer of functional virus
  • Flexible and versatile Gateway recombination cloning technology
  • Vector includes UbC promoter and V5 epitope tag at C terminus

Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Viral Gene Delivery

TRUSTED_SUSTAINABILITY

Specifications

Constitutive or Inducible System Constitutive
Delivery Type Lentiviral
Promoter UbC
Product Type Lentiviral Expression Kit
Selection Agent (Eukaryotic) Blasticidin
Content And Storage Vector kit (Boxes 1 and 2)
pLenti6⁄UbC⁄V5-DEST™ (Box 1): 6 μg, -20°C
pLenti6⁄UbC⁄V5-GW⁄lacZ Control vector (Box 1): 10 μg, -20°C
One Shot™ Stbl3™ Chemically Competent E. coli (Box 2): 21 x 50 μL, -80°C
pUC19 control DNA (10 pg⁄μL): 50 μL, -80°C
S.O.C. Medium: 6 mL -80°C

Support kit (Box 3)
ViraPower™ Packaging Mix (1 μg⁄μL): 195 μg, -20°C
Lipofectamine™ 2000: 0.75 mL, +4°C
Blasticidin: 50 mg, -20°C
293 FT cells (3 x 106⁄mL) (Box 4): 1 mL, liquid nitrogen
Protein Tag V5 Epitope Tag
Cloning Method Gateway
Quantity 1 kit
Vector pLenti
Product Line Gateway™, ViraPower™
For Use With (Application) Viral Expression
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For Research Use Only. Not for use in diagnostic procedures.

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