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Invitrogen™ pBudCE4.1 Mammalian Expression Vector

Product Code. 11510856
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Quantity:
20 μg
Unit Size:
20µg
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11510856 20 μg 20µg
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Product Code. 11510856 Supplier Invitrogen™ Supplier No. V53220

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Description

Includes

20μg each of pBudCE4.1 and pBudCE4.1/lacZ/CAT positive control vectors are supplied supercoiled and lyophilized.

Designed for the independent expression of two genes from a single plasmid in mammalian cells

  • Using pBudCE4.1 to generate stable mammalian expression cell lines ensures that there is an equivalent copy number of each gene in cell; this can eliminate variable expression due to differences in gene copy number
  • Provides expression cassettes with the CMV promoter for high-level transcription of genes with optional c-myc epitope tag for rapid detection and 6xHis sequence for simple purification
  • Human EF-1α promoter for high-level expression of genes with optional V5 epitope tag for rapid detection and 6xHis sequence for simple purification
  • Sh ble (ZeoR) gene for efficient selection in both mammalian cells and E. coli with selection agent Zeocin™

Constitutive Expression, Mammalian Expression, Multi-Gene Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

TRUSTED_SUSTAINABILITY

Specifications

Constitutive or Inducible System Constitutive
Delivery Type Transfection
Promoter EF-1α, CMV
Product Type Mammalian Expression Vector
Selection Agent (Eukaryotic) Zeocin™
Content And Storage 20 μg each of pBudCE4.1 and pBudCE4.1/lacZ/CAT positive control vectors are supplied supercoiled and lyophilized. Store at -20°C. Vectors are guaranteed stable for 6 months when properly stored.
Protein Tag His Tag (6x), V5 Epitope Tag, c-Myc Epitope Tag
Cloning Method Restriction Enzyme/MCS
Quantity 20 μg
Vector pBud
For Use With (Application) Multi-Gene Expression
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For Research Use Only. Not for use in diagnostic procedures.

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