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Invitrogen™ pGlow TOPO™ TA Expression Kit
Description
Includes
pGlow TOPO TA Expression Kits contains two boxes. The TOPO TA Cloning box contains linearized and topoisomerase I-activated pGlow-TOPO vector, dNTPs, primers for sequencing, salt solution, and control PCR template and primers. The box contains TOP10 E. coli cells, S.O.C. medium, and a supercoiled control plasmid.
- Cycle 3 GFP is exellent for noninvasive analysis of promoter elements within intact, living cells Fluorescent property of Cycle 3 GFP allows
- in vivo detection in virtually any cell type or species using microscopy with wild-type GFP filter sets or by fluorescence-activated cell sorting methods
Cell Analysis, Cell-Based Reporter Assays, Cellular Imaging, Enzyme and Protein Activity Assays, Fluorescent Protein (e.g. GFP) Assays, Fluorescent Protein Assays (GFP)/Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Reporter Assays
Specifications
Specifications
| Delivery Type | Transfection |
| Promoter | None (Promoterless) |
| Product Type | TOPO TA Expression Kit |
| Reporter Gene | GFP (Cycle 3) |
| Selection Agent (Eukaryotic) | Geneticin™ (G-418) |
| Content And Storage | pGlow TOPO™ TA Expression Kits contains two boxes. The TOPO™ TA Cloning box contains linearized and topoisomerase I-activated pGlow-TOPO™ vector, dNTPs, primers for sequencing, salt solution, and control PCR template and primers; store at -20°C. The One Shot™ box contains One Shot™ TOP10 E. coli cells, S.O.C. medium, and a supercoiled control plasmid; store at -80°C. The kits are guaranteed stable for 6 months when properly stored. |
| Protein Tag | Untagged |
| Cloning Method | TOPO-TA |
| Quantity | 20 reactions |
| Vector | pGlow, TOPO-TA Vectors |
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For Research Use Only. Not for use in diagnostic procedures.
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