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Invitrogen™ CellsDirect™ One-Step qRT-PCR Kit with ROX, for ABI 7000, 7300, 7700, and 7900(HT) instruments
Beschreibung
The CellsDirect One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.
Streamline Your Workflow
In traditional real-time quantitative RT-PCR (qRT-PCR), you first isolate RNA from cells in a time-consuming procedure that can lead to loss of material. Using the CellsDirect One-Step qRT-PCR Kit, you lyse the cells and add the complete lysate directly to a one-step qRT-PCR reaction with minimal handling and sample loss.
Ideal for Your qRT-PCR Application
CellsDirect technology is validated for gene expression studies involving limited samples, and is suitable for validation of RNAi knockdowns. CellsDirect One-Step qRT-PCR Kits are especially valuable for high-throughput applications by eliminating costly, time-consuming RNA purification steps. The CellsDirect One-Step qRT-PCR Kit may also be used to detect genomic DNA targets.
CellsDirect One-Step qRT-PCR Kits may be used in conjunction with hydrolysis probes (e.g., TaqMan™) or LUX™ fluorogenic primers.
- Efficiency: Offers significant time and cost savings for increased throughput
- Convenience: Enzyme mix includes both SuperScript™ III Reverse Transcriptase and Platinum™ Taq DNA Polymerase
- Sensitivity: No separate RNA isolation step increases sensitivity by minimizing sample loss
- Broad Detection Range: Detect targets from samples ranging from a single cell to 10,000 cells
- Versatility: Compatible with a wide variety of cell lines and samples collected by Laser Capture Microdissection (LCM)
- For use with ABI 7000, 7300, 7700, and 7900(HT) instruments
This kit includes 2X Reaction Mix containing ROX™ Reference Dye. An equivalent kit containing a separate tube of ROX Reference Dye, is also available.
Bestellinformation
Shipping Condition: Dry ice
Spezifikation
Spezifikation
| Concentration | 2X |
| Content And Storage | • Resuspension Buffer, 10 mL • Lysis Enhancer, 1 mL • 1 U/μL DNase I, Amplification Grade, 500 μL • 10X DNase I Buffer, 160 μL • 25 mM EDTA, 400 μL • SuperScript™ III RT/Platinum™ Taq Mix, 100 μL • 2X Reaction Mix with ROX™, 2 x 1.25 mL • 50 mM MgSO4, 1 mL • DEPC-treated water, 2 mL • 10 ng/μL HeLa Total RNA, 10 μL The kit is shipped on dry ice and should be stored at -20°C. |
| Detection Method | Primer-probe |
| Reaction Speed | Fast or Standard |
| For Use With (Equipment) | 7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System, Eppendorf™ MasterCycler™ RealPlex, QuantStudio 3 and 5 systems, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
| Product Line | CellsDirect, Platinum, SuperScript |
| Quantity | 100 reactions |
| For Use With (Application) | RT-PCR |
| No. of Reactions | 100 Reactions |
| Sample Type | Cells |
For Research Use Only. Not for use in diagnostic procedures.
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