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  7. Invitrogen™ CellsDirect™ One-Step qRT-PCR Kit with ROX, for ABI 7000, 7300, 7700, and 7900(HT) instruments 

Invitrogen™ CellsDirect™ One-Step qRT-PCR Kit with ROX, for ABI 7000, 7300, 7700, and 7900(HT) instruments

Product Code. 10583513
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Product Code. No. of Reactions unitSize
10583513 500 Reactions 500 reactions
10504863 100 Reactions 100 reactions
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Product Code. 10583513 Supplier Invitrogen™ Supplier No. 11754500

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Description

The CellsDirect One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.

The CellsDirect One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.

Streamline Your Workflow
In traditional real-time quantitative RT-PCR (qRT-PCR), you first isolate RNA from cells in a time-consuming procedure that can lead to loss of material. Using the CellsDirect One-Step qRT-PCR Kit, you lyse the cells and add the complete lysate directly to a one-step qRT-PCR reaction with minimal handling and sample loss.

Ideal for Your qRT-PCR Application
CellsDirect technology is validated for gene expression studies involving limited samples, and is suitable for validation of RNAi knockdowns. CellsDirect One-Step qRT-PCR Kits are especially valuable for high-throughput applications by eliminating costly, time-consuming RNA purification steps. The CellsDirect One-Step qRT-PCR Kit may also be used to detect genomic DNA targets.

CellsDirect One-Step qRT-PCR Kits may be used in conjunction with hydrolysis probes (e.g., TaqMan™) or LUX™ fluorogenic primers.

  • Efficiency: Offers significant time and cost savings for increased throughput
  • Convenience: Enzyme mix includes both SuperScript™ III Reverse Transcriptase and Platinum™ Taq DNA Polymerase
  • Sensitivity: No separate RNA isolation step increases sensitivity by minimizing sample loss
  • Broad Detection Range: Detect targets from samples ranging from a single cell to 10,000 cells
  • Versatility: Compatible with a wide variety of cell lines and samples collected by Laser Capture Microdissection (LCM)
  • For use with ABI 7000, 7300, 7700, and 7900(HT) instruments

This kit includes 2X Reaction Mix containing ROX™ Reference Dye. An equivalent kit containing a separate tube of ROX Reference Dye, is also available.

Order Info

Shipping Condition: Dry ice

TRUSTED_SUSTAINABILITY

Specifications

Concentration 2X
Content And Storage • Resuspension Buffer, 10 mL
• Lysis Enhancer, 1 mL
• 1 U/μL DNase I, Amplification Grade, 2 x 1.25 mL
• 10X DNase I Buffer, 800 μL
• 25 mM EDTA, 2 x 1 mL
• SuperScript™ III RT/Platinum™ Taq Mix, 500 μL
• 2X Reaction Mix with ROX™, 12.5 mL
• 50 mM MgSO4, 1 mL
• DEPC-treated water, 12.5 mL
• 10 ng/μL HeLa Total RNA, 10 μL

The kit is shipped on dry ice and should be stored at -20°C.
Detection Method Primer-probe
Reaction Speed Fast or Standard
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System, Eppendorf™ MasterCycler™ RealPlex, QuantStudio 3 and 5 systems, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Product Line CellsDirect, Platinum, SuperScript
Quantity 500 reactions
For Use With (Application) RT-PCR
No. of Reactions 500 Reactions
Sample Type Cells

For Research Use Only. Not for use in diagnostic procedures.

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