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Invitrogen™ pPICZ A, B, & C Pichia Vectors
Beschreibung
Beinhaltet
The EasySelect PichiaExpression Kit includes 20μg each of pPICZ A, B, and C and pPICZ A, B, and C, Pichia pastorisstrains X-33 (wild-type), GS115 (his4), KM71H (arg4 aox1::ARG4), and control strains for both intracellular and secreted expression, 250mg Zeocin , media (YP and YNB), EasyComp PichiaTransformation Kit, 2μg each 5' AOX1, 3' AOX1,and α-factor sequencing primers. A lacZ positive expression control is provided with pPICZ.
- These vectors contain Zeocin™ resistance gene for direct selection of multi-copy integrant strains
- By selecting with increasing amounts of Zeocin, strains with multiple copies of the gene of interest integrated into genome are obtained
- Increasing number of copies of gene of interest in recombinant Pichiastrain can result in higher expression levels
- Inducible AOX1promoter for high-level expression in Pichia pastoris c-mycepitope tag for convenient detection with Anti-mycAntibody
- C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with Anti-His(C-term) Antibody
- The pPICZ vector also features secretion signal for efficient transport of proteins to medium
Pichia pastoris Expression Systems, Protein Expression, Proteins, Expression, Isolation and Analysis, Yeast Expression
Spezifikation
Spezifikation
| Promoter | AOX1 |
| Product Type | Pichia Expression Vector |
| Content And Storage | 20 μg each of pPICZ A, B, & C suspended in 10 mM Tris–HCl, 1 mM EDTA, pH 8.0. A GS115/pPICZ/lacZ Positive Control strain is also included. Store vectors at -5 to -30°C. |
| Antibiotic Resistance Bacterial | Zeocin™ (ZeoR) |
| Protein Tag | His Tag (6x), c-Myc Epitope Tag |
| Cloning Method | Restriction Enzyme/MCS |
| Quantity | 20 μg |
| Vector | pPIC |
For Research Use Only. Not for use in diagnostic procedures.
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