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Invitrogen™ PureLink™ HiPure Plasmid Filter Maxiprep Kit
The PureLink HiPure Plasmid Filter Midiprep Kit is designed to isolate plasmid DNA of the highest purity from 25 to 100 mL of overnight bacterial culture.
Brand: Invitrogen™ K210016
195.34 EUR valid until 2024-12-14
Use promo code "21632" to get your promotional price.
Alert:
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”21632” to get your promotional price
Includes: The PureLink HiPure Plasmid Filter Kits include Equilibration Buffer (EQ1), Resuspension Buffer (R3), Lysis Buffer (L7), Precipitation Buffer (N3), RNase A, Wash Buffer (W8), Elution Buffer (E4), TE Buffer (TE), and HiPure Filter Columns.
Description
The PureLink HiPure Plasmid Filter Midiprep Kit is designed to isolate plasmid DNA of the highest purity from 25 to 100 mL of overnight bacterial culture. The PureLink HiPure Plasmid Filter Kits include HiPure Filter Columns, which combine filtration for clarification of bacterial lysate with anion-exchange resin purification of plasmid DNA, in single unit. The resulting plasmid DNA is of quality equivalent to that obtained by purification with two passes through cesium chloride gradients—the most rigorous method for DNA purification.
- Purify high-quality DNA in Less than two hours for transfections and other applications with no need for additional steps to remove contaminants like RNA, proteins, or endotoxins
- Additionally, elimination of phenol, chloroform, ethidium bromide, and cesium chloride from protocol minimizes exposure to and disposal of hazardous materials
PureLink HiPure Plasmid Filter Kits provide:
- Simple protocols—bacterial lysate clarification without centrifugation
- Fast results save time by purifying plasmid DNA in less than two hours
- High yields up to 200 μg for midipreps and up to 850 μg for maxipreps
- Reliable performance, high-quality plasmid DNA purified with these kits is suitable for wide variety of applications
How it works
- Addition of a HiPure Filter Column to the PureLink HiPure Plasmid Kits provides rapid clearing of the bacterial lysate without the need for centrifugation
- Lysate filtration cartridge is integrated into DNA-binding column for a combined step of lysate clarification and plasmid DNA binding directly to the anion-exchange resin
- Single column wash removes impurities such as RNA, proteins, metabolites, and other low molecular weight molecules; the ultrapure plasmid DNA is then eluted with high-salt buffer
- DNA is then desalted and concentrated with an isopropanol precipitation step, then collected by centrifugation
Order Info
Shipping Condition: Room Temperature
Specifications
| Filter Column | |
| Column | |
| Bacterial Culture | |
| 15 mL | |
| Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription | |
| 10 Preps | |
| > 200 μg (Large-Scale) Plasmid DNA | |
| Plasmid Filter MaxiPrep Kit | |
| Transfection Grade | |
| Plasmid DNA | |
| 90 min. |
| The PureLink™ HiPure Plasmid Filter Kits include Equilibration Buffer (EQ1), Resuspension Buffer (R3), Lysis Buffer (L7), Precipitation Buffer (N3), RNase A, Wash Buffer (W8), Elution Buffer (E4), TE Buffer (TE), and HiPure Filter Columns. Store all components at room temperature upon receipt. | |
| Anion Exchange Resin | |
| Maxi | |
| Plasmid DNA | |
| Not High-throughput Compatible (Manual) | |
| <40kb, Low Copy Plasmid, High Copy Plasmid, BAC | |
| PureLink™ | |
| 10 Preps | |
| Room Temperature | |
| PureLink™ | |
| 1000 μg |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- PureLink HiPure Plasmid Filter Maxiprep Kit
Signal Word
- Warning
Hazard Category
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
Hazard Statement
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
Precautionary Statement
- P264-Wash hands thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P362+P364-Take off contaminated clothing and wash it before reuse.
Supplemental information
- MIXTURE LIST-Contains : Sodium lauryl sulfate, Sodium hydroxide,acetic acid
For Research Use Only. Not for use in diagnostic procedures.