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  6. Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ INVαF' Chemically Competent E. coli 

Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ INVαF' Chemically Competent E. coli

Product Code. 10573263
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No. of Reactions:
20 Reactions
40 Reactions
Vector:
pCR2.1
pCRII
Unit Size:
Each
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Product Code. No. of Reactions Vector unitSize
10573263 40 Reactions pCR2.1 Each
10646533 20 Reactions pCRII Each
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Product Code. 10573263 Supplier Invitrogen™ Supplier No. K200040

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Description

Quick, one-step cloning strategy for directly inserting Taq-amplified PCR product into plasmid vector

The TA Cloning Kit uses the pCR2.1 cloning vector and ExpressLink T4 DNA Ligase to generate a ligation product in a 15-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts.

  • Fast and convenient: 15-minute, room-temperature ligation
  • Efficient: blue/white screening and >80% clones with correct insert
  • Flexible: choice of kanamycin or ampicillin resistance for flexible antibiotic selection
  • Hassle-free: eliminates any enzymatic modifications of PCR product
  • Streamlined: does not require use of PCR primers that contain restriction sites
  • 3'-T overhangs for direct ligation of Taq-amplified PCR products
  • T7 promoter for in vitro RNA transcription and sequencing
  • Versatile polylinker with flanking EcoR I sites for easy excision of inserts
  • M13 forward and reverse primer sites for sequencing

  • Taq polymerase has non-template-dependent activity that adds single deoxyadenosine (A) to the 3' ends of PCR products
  • Linearized vector supplied in has single 3' deoxythymidine (T) residues
  • Allows PCR inserts to ligate efficiently with vector

Cloning, PCR Cloning

TRUSTED_SUSTAINABILITY

Specifications

Bacterial or Yeast Strain INVαF ́
Cell Type Chemically Competent E. coli
Cloning Method TA Cloning
Content And Storage TA Cloning™ kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. The competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid.

Store One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Format Kit
For Use With (Application) PCR Cloning
No. of Reactions 40 Reactions
Product Line One Shot
Product Type Cloning Kit
Promoter T7
Quantity 40 reactions
Vector pCR2.1
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Safety and Handling

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Product Identifier
  • TA Cloning Kit, with pCR 2.1 Vector and One Shot INV
 Signal Word
  • Warning
 Hazard Category
  • Reproductive toxicity Category 2
  • Skin sensitiser Category 1
 Hazard Statement
  • H317-May cause an allergic skin reaction.
  • H361-Suspected of damaging fertility or the unborn child.
 Precautionary Statement
  • P201-Obtain special instructions before use.
  • P202-Do not handle until all safety precautions have been read and understood.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P272-Contaminated work clothing should not be allowed out of the workplace.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
  • P362+P364-Take off contaminated clothing and wash it before reuse.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : 2-Mercaptoethanol, Hydrogen chloride

For Research Use Only. Not for use in diagnostic procedures.

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