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Invitrogen™ TOPO™ TA Cloning™ Kit, with pCR™2.1-TOPO™, One Shot Mach1™-T1R Chemically Competent E. coli, and PureLink™ Quick Plasmid Miniprep Kit
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Quantity:
25 Reactions
Unit Size:
Each
Description
Includes
Topoisomerase I-activated pCR™2.1-TOPO™ vector; PCR buffer; Salt solution; dNTPs; Control template; M13 forward and reverse primers; Control PCR primers; Sterile water; One Shot™ Chemically Competent or Electrocomp™ E. coli; S.O.C. medium; Supercoiled pUC19 control plasmid
Uses a pCR™-TOPO Vector with covalently bound topoisomerase I for fast cloning and recombinants.
- 3'-T overhangs for direct ligation of Taq-amplified PCR products
- Choice of T7 (pCR2.1-TOPO™) or T7 and SP6 (pCRII-TOPO™) promoters for in vitro RNA transcription and sequencing. Each vector also contains M13 forward and reverse primer sites for sequencing.
- EcoR I sites flanking the PCR product insertion site for easy excision of inserts
- Kanamycin and ampicillin resistance genes for your choice of selection in E. coli
- Easy blue/white colony screening for selection of recombinants
- TOPO TA Cloning Kits are available in combo kits combined with a PureLink™ Quick Plasmid Miniprep Kit (50 preps) for fast plasmid purification of your TOPO-cloned inserts for downstream analysis
Cloning, PCR Cloning
Specifications
Specifications
| Bacterial or Yeast Strain | Mach1™ |
| Cell Type | Chemically Competent |
| Cloning Method | TOPO™-TA |
| Content And Storage | Box 1: • Topoisomerase I-activated pCR™2.1-TOPO™ vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid Store at -68 to -85°C. |
| Format | Kit |
| Product Line | One Shot™ |
| Product Type | Cloning Kit |
| Promoter | T7, SP6 |
| Quantity | 25 Reactions |
| Vector | TOPO-TA Cloning Vectors |
For Research Use Only. Not for use in diagnostic procedures.
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