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Thermo Scientific™ Klenow Fragment, exo– (5 U/μL)
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Description
Klenow Fragment, exo-, is the large fragment of DNA polymerase I . It exhibits 5'→3' polymerase activity, but lacks the 3'→5' and 5'→3' exonuclease activities of DNA Polymerase I. The 3'→5' exonuclease activity of the enzyme is eliminated by mutations in the 3'→5'-exonuclease active sit.
Highlights
- Lacks 3'→5' exonuclease activity
- Incorporates modified nucleotides (e.g., Cy3-, Cy5-, fluorescein-, rhodamine-, aminoallyl-, biotin-labeled nucleotides)
- Active in restriction enzyme, PCR, and RT buffers
Applications
- Random-primed DNA labeling
- Labeling by fill-in 5'-overhangs of dsDNA
- Strand displacement amplification (SDA)
- DNA sequencing by the Sanger method
Note
Klenow Fragment, exo- is not recommended for DNA blunting reactions prior to DNA ligation since it frequently adds one or more extra nucleotides to the 3'-terminus of blunt-end DNA substrates in a non-template directed fashion
Specifikationer
Specifikationer
| Concentration | 5 U/μL |
| Polymerase | DNA Polymerase I |
| Compatible Buffer | Restriction Enzyme, PCR, RT Buffer |
| Quantity | 300 units |
| Product Type | Klenow Fragment |
For Research Use Only. Not for use in diagnostic procedures.
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