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Invitrogen™ Multispecies Leukotriene B4 Competitive ELISA Kit

Product Code. 10753115
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Quantity:
96 Tests
Unit Size:
1 set
This item is not returnable. View return policy

Product Code. 10753115

Brand: Invitrogen™ EHLTB4

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559.36 EUR valid until 2025-12-31
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This item is not returnable. View return policy

Description

ELISA

The Multispecies Leukotriene B4 (LTB4) ELISA quantitates LTB4 in human saliva,urine,porcine plasma and tissue culture medium.

Principle of the method

This LTB4 competitive ELISA kit is an immunoassay for the quantitative determination of leukotriene B4 (LTB4) in biological fluids. The assay is based on the competition between LTB4 in the standard or sample and alkaline phosphatase conjugated LTB4 (LTB4-AP) for a limited amount of LTB4 monoclonal antibody bound to an anti-Rabbit IgG precoated 96-well plate. As the concentration of LTB4 in the sample increases,the amount of LTB4-AP captured by the coating antibody decreases. Thus,there is an inverse relationship between optical density (OD) and the amount of analyte in the sample.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.

Leukotriene B4 (LTB4) is a leukotriene involved in inflammation. It is produced from leukocytes in response to inflammatory mediators and is able to induce the adhesion and activation of leukocytes on the endothelium, allowing them to bind and cross into the tissue. In neutrophils, LTB4 is a potent chemoattractant, and is able to induce the formation of reactive oxygen species and the release of lysosomal enzymes.
TRUSTED_SUSTAINABILITY

Specifications

Assay Range 11.7 to 120,000 pg/mL
Assay Sensitivity <5.63 pg/mL
Description LTB4 Competitive ELISA Kit
Conjugate AP
Immunoassay Kit Format Colorimetric ELISA (p-NPP substrate) with Plates
Product Type ELISA
Sample Type Plasma, Saliva, Supernatant, Urine
Specificity No significant cross-reaction with PGE2, PGF2a, 20-OH-LTB4, 20-COOH-LTB4, LTC4, LTD4, LTE4, 5(S)-HETE, 12(S)-HETE, 15(S)-HETE; less than 1% cross-reaction with 12-epi-LTB4; approx. 5% cross-reaction with 6-trans-LTB4 and 6-trans-12-epi-LTB4
Sufficient For 96 microplate wells
Target Species Human, Porcine
For Use With (Equipment) Colorimetric Microplate Reader
Interassay CV 12.4%
Intraassay CV 6.5
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent, Detection Antibody, AP Conjugate, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye AP
Measurement Absorbance at 405 nm minus absorbance (at 570 nm)
Precision Dependent upon sample type and level
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Saliva 25 μL, Supernatant 100 μL, Urine 100 μL
Storage Requirements -20°C
Target Leukotriene B4
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs
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