Thermo Scientific™ Maxima Reverse Transcriptase (200 U/μL)

Maxima Reverse Transcriptase is capable of reproducible cDNA synthesis from a wide range of input total RNA amounts (from 1 pg to 5 μg) at elevated temperatures (50–65°C), which makes this enzyme an ideal tool for two step RT-qPCR (see supporting data).

Due to its high thermostability, Maxima enzyme maintains full activity during the entire reverse transcription reaction, generates the highest yields of cDNA, and is able to synthesize even very long RNA transcripts up to 20 kb. The reaction temperature can be increased up to 65°C for efficient transcription of RNA regions with a high secondary structure or to improve specificity using gene specific primers. Due to its increased synthesis rate, the reverse transcriptase reaction can be completed in 15 to 30 minutes.

Features of Maxima Reverse Transcriptase include:

• High yields of full-length cDNA up to 20 kb
• Active up to 65°C
• Thermostable—90% active after incubation at 50°C for 60 minutes
• High sensitivity allows reproducible cDNA synthesis from a wide range of total RNA quantities (1 pg to 5 μg)
• Efficient formula for complete cDNA synthesis in 15 to 30 minutes
• Incorporates modified nucleotides

Applications

• Full-length cDNA synthesis for long transcripts and ORFs
• Reverse transcription at elevated temperatures to reduce effects of secondary structure
• cDNA cloning and construct generation
• RNA-seq & single cell RNA-seq library prep
• Isoform and transcript characterization
• Template-switching-based full-length RNA-seq

Also available

• Maxima Reverse Transcriptase (Cat. No. EP0741)
• Maxima First Strand cDNA Synthesis Kit for RT-qPCR (Cat. No. K1641)
• Maxima First Strand cDNA Synthesis Kit for RT-qPCR, with dsDNase (Cat. No. K1671)