missing translation for 'onlineSavingsMsg'
Learn More

Invitrogen™ MegaScript™ T7 Transcription Kit Plus

The MegaScript T7 Transcription Kit Plus contains all the reagents and buffers necessary for in vitro synthesis of large amounts of RNA.

599.00 € - 8682.00 €

Specifications

Cell Type HEK293, 293, HeLa, CHO-K1, CHO, Jurkat, Primary Human T Cells, Primary Cells, Stem Cells, iPSC, NIH-3T3, HepG2, A549, HUVEC
Final Product Type In vitro Transcribed RNA
Format Kit
Isolation Technology Silica Spin Column
Purification Target In vitro Transcribed RNA
View More Specs

Products 2
Product Code Brand No. of Reactions Price Quantity & Availability  
Product Code Brand No. of Reactions Price Quantity & Availability  
17984681
View Documents Promotion Details
Invitrogen™
A57622
50 Reactions
599.00 €
50 reactions
Please to purchase this item. Need a web account? Register with us today!
17934741
View Documents Promotion Details
Invitrogen™
A57623
1000 Reactions
8682.00 €
1000 reactions
Please to purchase this item. Need a web account? Register with us today!
Description

Description

The MegaScript T7 Transcription Kit Plus contains all the reagents and buffers necessary for in vitro synthesis of large amounts of RNA. Each 20-μL reaction yields an average of 100 μg of RNA, while the scaled-up protocol yields up to 1 mg of RNA. This kit is suitable for full- or partially-modified-nucleotide substitution to produce modified RNA. It is also suitable for co-transcriptional capping with cap analogs to make capped RNA that can be translated into protein.

Features of the MegaScript T7 Transcription Kit Plus include:
• Optimized to achieve high mRNA yields (>5 mg/mL)
• Flexible—allows use of modified nucleotides and/or cap analogs such as ARCA or CleanCap
• Includes GFP control template
• Made up of high quality reagents (TheraPure)
• Available in two sizes: 50 and 1000 reactions

The MegaScript T7 Transcription Kit Plus requires a linearized DNA template with a T7 RNA polymerase promoter to create >5 mg/mL of RNA. MEGAscript kits are intended for the synthesis of large amounts of unlabeled or low-specific-activity RNA for a variety of uses, including transfections, preclinical mRNA therapeutic mRNA studies, microinjections, in vitro translation, and RNA structure and function analysis. Note that this kit does not create capped RNA, which is necessary for translational studies. MEGAscript kits are not recommended for synthesis of high-specific-activity probes.

Specifications

Specifications

HEK293, 293, HeLa, CHO-K1, CHO, Jurkat, Primary Human T Cells, Primary Cells, Stem Cells, iPSC, NIH-3T3, HepG2, A549, HUVEC
Kit
In vitro Transcribed RNA
In vitro Transcribed RNA
Silica Spin Column
In vitro Transcribed RNA, Total RNA
Videos
SDS
Documents

Documents

Product Certifications

For Research Use Only.