IBA Lifesciences™ MHC I-Strep H-2 Db; gp33 LCMV (KAVYNFATM); Fused to Twin-Strep-tag™

MHC I-Strep H-2 Db; gp33 LCMV (KAVYNFATM) is a recombinantly expressed MHC Class I molecule that presents a peptide from a lymphocytic choreomeningitis virus (LCMV) glycoprotein 33 (gp33). Our MHC I Streptamer™ approach is based on our Strep-tag™ technology and facilitates reversible antigen-specific T cell labeling. A Twin-Strep-tag™ fused to the MHC I-Strep permits multimerization of MHC I complexes on a Strep-Tactin™ backbone, resulting in the formation of MHC I Streptamers.

For multimerizing MHC I-Streps, Strep-Tactin™ TACS Agarose columns, Strep-Tactin™ Magnetic Microbeads or fluorophore-conjugated Strep-Tactin™ can be used. This allows affinity chromatographic or magnetic isolation or cell staining for flow cytometry analysis and sorting (FACS), depending on the experimental requirements.

• Addition of biotin reverses multimerization of MHC Class I molecules, leading to dissociation from target cells
• Resulting label-free cells are highly viable and fully functional