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Invitrogen™ ImaGene Red™ C12RG lacZ Gene Expression Kit

Product Code. 10709774
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Product Code. 10709774 Supplier Invitrogen™ Lieferanten-Nr. I2906

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Beschreibung

Substrates cleaved within cell for fluorescent product

The ImaGene Red™ lacZ Gene Expression Kit contains a fluorescein-based galactosidase substrate that has been covalently modified to include a 12-carbon lipophilic moiety, C12RG. Once inside the cell, the substrates are cleaved by β-galactosidase, producing a fluorescent product that is well retained by the cells, probably by incorporation of the lipophilic tail within the cellular membrane. In addition to the C12RG, the kits also include the broad-spectrum β-galactosidase inhibitor, PETG, and chloroquine diphosphate for inhibiting acidic hydrolysis of the substrate.

Beta-Galactosidase Assays, Cell Analysis, Cellular Imaging, Enzyme and Protein Activity Assays, Immunofluorescence (IF), Immunofluorescence Staining and Detection, Proteins, Expression, Isolation and Analysis, Reporter Assays

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Shipping Condition: Room Temperature

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Spezifikation

Product Type β-Galactosidase Reporter Gene Assay System
Detection Method Fluorescence
Assay β-Gal (lacZ) Assay
Content And Storage Store in freezer (-5 to -30°C) and protect from light.
Shipping Condition Room Temperature
Substrate C12RG
Substrate Type Beta-Gal Substrate
Substrate Properties Chemical Substrate
Target Enzyme Beta-Galactosidase
Format Kit
Product Line DetectaGene
Quantity 1 kit
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For Research Use Only. Not for use in diagnostic procedures.

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