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Invitrogen™ OxyBURST™ Green H2DCFDA, SE

Product Code. 11510286
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5 mg
Unit Size:
5mg
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11510286 5 mg 5mg
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Product Code. 11510286 Supplier Invitrogen™ Supplier No. D2935
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Description

Amine-reactive assay reagent

OxyBURST™ Green H2DCFDA succinimidyl ester (2',7'-dichlorodihydrofluorescein diacetate, SE) is an amine-reactive assay reagent that can be used to prepare oxidation-sensitive conjugates of a wide variety of biomolecules and particles, including antibodies, antigens, peptides, proteins, dextrans, bacteria, yeast and polystyrene microspheres. Following conjugation to amines, the two acetates of OxyBURST™ Green H2DCFDA can be removed by treatment with hydroxylamine at neutral pH to yield the dihydrofluorescein conjugate. OxyBURST™ Green H2DCFDA conjugates are nonfluorescent until they are oxidized to the corresponding fluorescein derivatives.

ROS Indicator Specifications:

  • Ex/Em: ∼492–495/517–527 nm
  • Product is air sensitive and should be stored under dry argon or nitrogen
  • Product may be dissolved in DMSO, DMF, or ethanol for use
  • Indicator is cell permeant (cell loading protocols are available in the literature)
  • Fluorescence can be monitored using a flow cytometer, fluorometer, microplate reader, or fluorescence microscope, using excitation sources and filters appropriate for fluorescein

Find More ROS Indicators

We offer an assortment of Molecular Probes™ products for the generation of reactive oxygen species (ROS), including singlet oxygen, superoxide, hydroxyl radical and various peroxide and hydroperoxides, as well as for their fluorometric detection in solution. Review Generating and Detecting Reactive Oxygen Species—Section 18.2 in the Molecular Probes™ Handbook for more information on these products.

For Research Use. Not for human or animal therapeutic or diagnostic use.

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Specifications

Quantity 5 mg
For Use With (Application) ROS
Detection Method Fluorescence
Product Type ROS Indicator
Excitation Wavelength Range 492 to 495 nm
Emission Wavelength Range 517 to 527 nm

Safety and Handling

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Product Identifier
  • 2',7'-DICHLORODIHYDROFLUO
 Signal Word
  • Warning
 Hazard Category
  • Acute toxicity Category 4
  • Reproductive toxicity Category 2
  • Skin sensitiser Category 1
 Hazard Statement
  • H302-Harmful if swallowed.
  • H317-May cause an allergic skin reaction.
  • H361-Suspected of damaging fertility or the unborn child.
 Precautionary Statement
  • P201-Obtain special instructions before use.
  • P264-Wash hands thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
 Supplemental information
  • MIXTURE LIST-Contain: 2'',7''-DICHLORODIHYDROFLUO

For Research Use Only. Not for use in diagnostic procedures.

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