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Invitrogen™ SYTO™ 40 Blue Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO

Product Code. 10514243
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Product Code. 10514243 Supplier Invitrogen™ Supplier No. S11351
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Description

For use with fluorescence microscopy and flow cytometry

The cell-permeant SYTO 40 blue fluorescent nucleic acid stain exhibits bright, blue fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary from one cell type to another, we offer the SYTO Blue Fluorescent Nucleic Acid Stain Sampler Kit (Cat. No. S-11350) to enable researchers to find the dye that works best for a particular application.

Any physiological buffer between pH 7.0–8.0, including PBS, can be used to dilute the SYTO dyes for the staining solution.

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Specifications

Color Red, Blue
Content And Storage Store in freezer at -5°C to -30°C and protect from light.
Excitation Wavelength Range 420 nm
Dye Type Cell-Permeant
For Use With (Equipment) Fluorescence Microscope
Quantity 250 μL
Volume (Metric) 250 μL
Detection Method Fluorescence
Description SYTO™ 40 Blue Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO
Emission 441 nm
Product Line SYTO
Shipping Condition Room Temperature
Label Type Fluorescent Dye
Product Type Nucleic Acid Stain
Sub Cellular Localization Nucleic Acids
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Frequently Asked Questions (FAQs)

How do SYTO dyes bind to DNA?

The binding mode of SYTO nucleic acid stains is unknown. However, the behavior of these and related nucleic acid dyes suggests the following binding properties:

1.They appear to contact the solvent (suggested by sensitivity to salt, divalent cations, and in particular, SDS) and thus are likely to have contacts in the grooves.
2.All SYTO dyes appear to show some base selectivity and are thus likely to have minor groove contacts.
3.They can be removed from nucleic acid via ethanol precipitation; this characteristic is not shared by ethidium bromide and other intercalators. Likewise, the dyes are not removed from nucleic acid via butanol or chloroform extraction. These extraction methods do remove ethidium bromide from nucleic acid. 4. SYTO binding is not affected by nonionic detergents.
5. SYTO dyes are not quenched by BrdU, so they do not bind nucleic acids in precisely the same way as Hoechst 33342 and DAPI ((4′,6-diamidino-2-phenylindole).

SYBR Green I has shown little mutagenicity on frameshift indicator strains, indicating that it isn't likely to strongly intercalate.

For Research Use Only. Not for use in diagnostic procedures.

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