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Description
Nitrocellulose is a popular binding matrix for western blotting because of its high affinity for proteins and compatibility with a variety of detection methods (western blotting, dot-blot assays, and other protein or nucleic acid methods). The 0.45-μm pore size is well-suited for a wide range of western blotting conditions and ideal for the transfer of most proteins (>20 kDa) and nucleic acids (>300 bp).
Features include:
• 100% pure nitrocellulose membrane
• Convenient—save time with pre-cut sheets or choose the roll format for the flexibility to cut to the ideal size
• Compatible with commonly used transfer conditions and detection methods such as staining, immunodetection, chemiluminescence, fluorescence, and radiolabeling
• High-binding affinity—provides excellent protein binding, blocks easily, and does not require pre-wetting with alcohol
• Low background: produce high-quality data with low background signal in both chemiluminescent and fluorescent western blotting
Specifications
Specifications
| Length (Metric) | 8 cm |
| Width (Metric) | 8 cm |
| Description | Nitrocellulose Membranes, 0.45 μm, 8 x 8 cm |
| Binding Capacity | ∼45–60 μg/cm2 of BSA |
| Quantity | 15 Pre-cut Membranes |
| Format | Sheet |
| Material | Nitrocellulose |
| Pore Size | 0.45 μm |
| Content And Storage | Store membranes flat at ambient temperature, away from chemical vapors. Some solvent vapors may partially dissolve the membranes, which will disrupt the pore structure. Keep membranes out of direct sunlight. Sunlight may cause the nitrocellulose to dry and become brittle. Note: Nitrocellulose membrane filters are highly flammable. Keep away from heat and open flame. Flash point is approximately 2°C. Membranes can be sterilized by steam-autoclaving at 121°C. |
| Shipping Condition | Room Temperature |
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Safety and Handling
- NITROCELLULOSE MEMBRANE, 0.45U
- Danger
- Flammable solid Category 1
- H228-Flammable solid.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P240-Ground/bond container and receiving equipment.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P370+P378-In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish.
- MIXTURE LIST-Contains : Nitrocellulose
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