missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Immunoassay Reagents and Kits
  4. Life Science Research ELISA Kits
  5. 10212423

Invitrogen™ Human Amyloid beta 42 ELISA Kit, Ultrasensitive

Product Code. 10212423
Click to view available options
Quantity:
96 Tests
Unit Size:
96 tests
This item is not returnable. View return policy

Product Code. 10212423

Brand: Invitrogen™ KHB3544

Please to purchase this item. Need a web account? Register with us today!

730.62 EUR valid until 2025-12-19
BEST PRICE on Promo! Use promo code "24110" to get your promotional price.
This item is not returnable. View return policy

Description

ELISA

The Human Amyloid Beta 42 Ultrasensitive (Hu Abeta 42 US) ELISA quantitates Hu Abeta 42 at ultra-low levels in humantissue culture medium, tissue homogenates and cerebrospinal fluid. The assay will exclusively recognize both natural and recombinant Hu Abeta 42 US. Principle of the method The Human Abeta 42 US solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Beta amyloid is a 40-43 amino acid peptide cleaved from amyloid precursor protein by a protease, gamma-secretase. Beta Amyloid (1-40) (Amyloid beta 40 or Abeta 40) together with beta Amyloid (1-42) (Amyloid beta 42 or Abeta 42) are two major C-terminal variants of the amyloid beta protein, constituting the majority of amyloid beta proteins. Both the Amyloid beta 40 and Amyloid beta 42 alloforms are major components of the extracellular plaques found in Alzheimer's disease brain tissue.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P05067
Assay Range 1.56 to 100 pg/mL
Assay Sensitivity <1 pg/mL
Concentration 1.56 to 100 pg/mL
Conjugate HRP
Product Type ELISA
Sample Type Cerebrospinal Fluid, Supernatant, Tissue Homogenates
Target Species Human
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 351
Gene Symbol AAA,ABETA,ABPP,AD1I,CTFgamma,CVAP,PN-II,PN2,APP
Incubation Time 4 hr.
Interassay CV 7.5%
Intraassay CV 8.6
Kit Contents Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Detection Antibody, Anti-IgG HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Marker Gene Aβ 42
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Cerebrospinal Fluid 25 μL, Supernatant 25 μL, Tissue Homogenate 25 μL
Storage Requirements 2°C to 8°C
Target Amyloid beta 42
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs
Show More Show Less
Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.