missing translation for 'onlineSavingsMsg'
Learn More

Invitrogen™ Dynabeads™ Protein A Immunoprecipitation Kit

Dynabeads Protein A Immunoprecipitation Kit is intended for immunoprecipitation (IP) and includes all reagents and buffers required to perform IP in combination with your own antibody.

Brand:  Invitrogen™

376.01 EUR valid until 2024-12-14
Use promo code "21632" to get your promotional price.


Product Code. 10442945

Please to purchase this item. Need a web account? Register with us today!

Explore more special offers

Alert:

To receive the discount customers must purchase three of the same product at list price in a single order to receive 33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”21632” to get your promotional price

This item is not returnable. View return policy

Description

Description

Dynabeads Protein A Immunoprecipitation Kit is intended for immunoprecipitation (IP) and includes all reagents and buffers required to perform IP in combination with your own antibody. The kit is optimized for standard IP, but can also be used for Co-IP, chromatin IP (ChIP), or RNA IP (RIP). The kit includes Dynabeads Protein A magnetic beads with recombinant Protein A (∼45 kDa) covalently coupled to the bead surface. Both manual and automated protocols are available.

Benefits of Dynabeads Protein A Immunoprecipitation Kit include:
Fast and easy—IP in less than 40 minutes with no columns, centrifugations, or time-consuming pre-clearing required
High performance—high target protein yield with low antibody consumption
Low background—very low non-specific binding with a high signal-to-noise ratio
High reproducibility—uniform beads and optimized buffers help ensure consistent results
Automation ready—automation protocols available with the KingFisher Sample Purification instruments

Gentle separation causes minimal physical stress to proteins
The magnetic separation technology utilized by Dynabeads Protein A beads is rapid and gentle, causing minimal physical stress to target proteins. This permits the isolation and concentration of labile composites that might otherwise dissociate or be damaged by proteases during long incubation times. Native protein conformation and large protein complexes are preserved.

Binding strength and antibody binding capacity
Dynabeads Protein A beads allow for the isolation of most mammalian immunoglobulins (Ig). The amount of Ig captured depends on the concentration of Ig in the starting sample and on the type and source of the Ig. 100 μL of Dynabeads Protein A will isolate approximately 25–30 μg human IgG from a sample containing 20–200 μg IgG/mL. Predominantly Fc-binding allows optimal Ig orientation.

Dynabeads magnetic beads are not porous in nature such as Sepharose or agarose resins. This means that all antibodies bind to the smooth outer surface of the beads and are available to bind to the target protein. This translates to a low antibody consumption while obtaining a high yield of the target protein. The smooth bead surface is also the reason for the low non-specific binding that Dynabeads magnetic beads are known for. The recombinant protein A on the beads does not contain albumin binding sites, thus albumin is not co-purified during the procedure

Dynabeads immunoprecipitation protocol is fast and easy
The manual IP protocol is simple and can be performed in less than 40 minutes with 3 easy bind-incubate-elute steps. The procedure does not require any pre-clearing due to the low non-specific binding and uses very little antibody while still obtaining a high IP yield, thus saving both time and cost per IP.

The antibody-coated beads can be used in a variety of IP applications such as Co-IP, chromatin IP (ChIP/ChIP sequencing), RNA IP (RIP, RIP sequencing), small-scale IgG purification, and protein purification. The isolated pure target protein can be used in downstream western blot, mass spectrometry analysis, sequencing, etc.

Automation-ready for use with KingFisher purification systems
The 2.8-μm magnetic beads are ideal for high-throughput enrichment. This process can be automated using any of our KingFisher sample purification systems or liquid handlers.

Commercial supply 
Our manufacturing sites are ISO 13485-certified. Bead characteristics and manufacturing conditions help ensure consistency and batch reproducibility making them an ideal choice for commercial supply. If you are in the process of customizing Dynabeads Protein A Immunoprecipitation Kit in a commercial product or service or need a larger bulk volume of this product, please contact us at oemdynal@lifetech.com or see our Dynabeads OEM page.

*Sepharose is a trademark of GE Healthcare Bio-Sciences AB.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

30 mg/mL
• 2 mL Dynabeads Protein A
• 16 mL Antibody (Ab) Washing and Binding buffer
• 28 mL Washing Buffer
• 1 mL Elution Buffer

Store at 2°C to 8°C.

KingFisher™ Sample Purification System, DynaMag™ magnets
Ambient Temperature
Dynabeads™
Co-immunoprecipitation
Protein A
Kit
ISO9001 and ISO13485
Magnetic Polystyrene
2.8 μm
Yes
Cell Cultures, Clinical Sample, Supernatants, Tissue Digests, Cell Extracts, Serum
2 mL
IP Kit
40 Tests
Brown
For Research Use Only
Product Suggestions

Product Suggestions

Videos
SDS
Documents

Documents

Certificates
Special Offers

Special Offers

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.

For Research Use Only. Not for use in diagnostic procedures.