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Invitrogen™ Human CD23 ELISA Kit

Product Code. 10262643
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Quantity:
96 Tests
Unit Size:
96 tests
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Product Code. 10262643

Brand: Invitrogen™ KAS0251

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Description

ELISA

The Human CD23 ELISA quantitates Hu CD23 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu CD23. Principle of the method The Human CD23 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CD23 is a 45 kDa glycoprotein which is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. The CD23 molecule is identical to the low affinity IgE receptor found on B cells. Expression of CD23 has been detected in neoplastic cells from cases of B cell chronic lymphocyctic leukaemia and some cases of centroblastic/centrocytic lymphoma. CD23 is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. Functionally, CD23 is involved in B cell growth and differentiation, and IgE production. Further, CD23 has a soluble form that is a potent mitogenic factor. Diseases associated with CD23 dysfunction include chronic conjunctivitis and chronic lymphocytic leukemia.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P06734
Assay Range 1 to 20 U/mL
Assay Sensitivity 0.15 U/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
Target Species Human
For Use With (Application) Colorimetric Detection
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 2208
Gene Symbol BLAST-2,CD23,CD23A,CLEC4J,FCE2,IGEBF
Incubation Time 2.5 hr.
Interassay CV 4.2%
Intraassay CV 3.2
Kit Contents Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Incubation Buffer, HRP conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Controls
Label or Dye HRP
Marker Gene CD23 (soluble)
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Target CD23
Test Time 1 hrs 20 mins
Total Assay Time 2 hrs 30 mins
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