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  5. Invitrogen™ Human FGF2 ELISA Kit 

Invitrogen™ Human FGF2 ELISA Kit

Product Code. p-4922208
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Quantity:
2 x 96 Tests
96 Tests
Unit Size:
192 tests
96 tests
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Product Code. 10797493

Brand: Invitrogen™ KHG0021

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554.80 EUR valid until 2025-12-31
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This item is not returnable. View return policy

Description

Includes

Pre-coated plate, standard, detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.

ELISA

The Human FGF-b ELISA Kit quantifies human Fibroblast Growth Factor basic (FGF-b) in serum, plasma, buffered solution, or cell culture medium. The assay will recognize both the natural 18 kDa isoform and recombinant FGF basic-146 and FGF basic-157. Principle of the method The Human FGF-b solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

FGF2 (FGFb, fibroblast growth factor basic) belongs to the fibroblast growth factor (FGF) family, and interacts with high-affinity transmembrane receptors to influence cell proliferation and tissue neovascularization. FGF2 exists as five isoforms with distinct intracellular localizations and functions. The 18 kDa isoform is predominantly cytosolic and acts through cell surface receptors, whereas the 22, 22.5, 24 and 34 kDa isoforms are nuclear and may signal independent of transmembrane receptor pathways. In humans, the gene is located on the q arm of chromosome 4. FGF2 has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for FGF2 contains multiple polyadenylation sites, and is alternatively translated from non-AUG and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. Diseases associated with FGF2 dysfunction include Kaposi Sarcoma and corneal neovascularization.

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Specifications

Accession Number P09038
Assay Range 15.6 to 1000 pg/mL
Assay Sensitivity 7 pg/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
Target Species Human
For Use With (Application) Colorimetric Detection
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 2247
Gene Symbol BFGF,FGF-2,FGFB,HBGF-2
Incubation Time 4 hr.
Interassay CV 5.5%
Intraassay CV 4.3
Kit Contents Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Marker Gene FGF-Basic
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target FGF2
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs
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Safety and Handling

compliance-icons
Product Identifier
  • Human FGF2 ELISA Kit
 Signal Word
  • Warning
 Hazard Category
  • Acute toxicity Category 4
  • LONG-TERM AQUATIC HAZARD Chronic 3
 Hazard Statement
  • H302-Harmful if swallowed.
  • H412-Harmful to aquatic life with long lasting effects.
 Precautionary Statement
  • P264-Wash hands thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P273-Avoid release to the environment.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P330-Rinse mouth.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : SODIUM AZIDE

For Research Use Only. Not for use in diagnostic procedures.

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