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  5. Macherey-Nagel™ NucleoBond™ PC EF, kit for endotoxin-free plasmid DNA

Macherey-Nagel™ NucleoBond™ PC EF, kit for endotoxin-free plasmid DNA

Product Code. 12860661
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No. of Reactions:
5
10
Yield:
100–500μg
2,000–10,000μg
500–2,000μg
Sample Volume:
30 - 150mL
150 - 500mL
500 - 2000mL
Test Time:
100 min./2 preps
150 min./2 preps
180 min./2 preps
Unit Size:
10 reactions
5 preps
5 reactions
This item is not returnable. View return policy

Product Code. 12860661

Brand: Macherey-Nagel™ 740549

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This item is not returnable. View return policy

Description

Removes endotoxins via an additional washing step, without ice incubation. Macherey-Nagel™ NucleoBond™ PC EF Kit is a classic anion exchanger for purification of endotoxin-free plasmid DNA. The kit includes NucleoBond™ AX Columns, all buffers, RNase A and NucleoBond™ Filters.

An important prerequisite for transfections and gene therapy applications is endotoxin-free plasmid DNA. The patented procedure of NucleoBond™ PC EF reduces endotoxins to a very low level using the same working procedure as the standard protocol. In comparison to competitive kits, there is no need for a time-consuming incubation step to remove the endotoxins.

Features

  • Fast and easy clarification by NucleoBond™ Folded Filters—No centrifugation required, no shearing forces
  • Proven anion-exchange technology—Yields transfection-grade high-copy plasmid DNA from E. coli
  • NucleoBond™ Columns contain NucleoBond™ AX Silica Resin packed between inert filters
  • Polypropylene columns are resistant to organic solvents and are free of DNase and RNase
  • AX Resin can be used over a wide pH range and can remain in contact with buffers for up to three hours

Specifications

  • DNA with less than 0.1 EU/μg DNA
  • Purity of plasmid DNA: A260/A280 = 1.80–1.95
  • Recovery of plasmid DNA >90%
  • Structural integrity >90%
  • Yield between 500μg up to 10mg, depending on kit used

Specifications

Concentration Endotoxin level: <0.1 EU/μg
Format Mega Gravity-flow Column
Isolation Technology Anion Exchange Chromatography Technology
Efficiency Recovery of plasmid DNA: >90%
For Use With (Application) High-copy plasmids from E. coli
Includes NucleoBond AX 2000 Columns, buffers, RNase A, NucleoBond Bottle Top Filters
No. of Reactions 5
Product Type NucleoBond™ PC 2,000 EF
Quantity 1 Kit
Purity A260/A280: 1.80–1.95
RNase Yes
Sample Volume 150 - 500mL
Size <300 kbp
Test Time 150 min./2 preps
Yield 500–2,000μg
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Safety and Handling

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Hazard Category
  • Flammable liquid Category 3
  • Substances and mixtures corrosive to metals Category 1
  • Skin corrosion/irritation Category 2
  • Respiratory sensitiser Category 1
  • Serious eye damage/eye irritation Category 2
  • Skin sensitiser Category 1
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