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  5. Macherey-Nagel™ NucleoBond™ Xtra Midi EF, Midi kit for endotoxin-free plasmid DNA

Macherey-Nagel™ NucleoBond™ Xtra Midi EF, Midi kit for endotoxin-free plasmid DNA

Product Code. 12773550
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No. of Reactions:
10
50
Unit Size:
10 reactions
50 reactions
This item is not returnable. View return policy

Product Code. 12773550

Brand: Macherey-Nagel™ 740420.10

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This item is not returnable. View return policy

Description

Purify endotoxin-free plasmid DNA with high yield. The NucleoBond™ Xtra Midi EF Kit represents the new generation of anion exchanger allowing high yield, ultra-fast.

Features
  • Endotoxin-free
  • Plasmid DNA with less than 0.05 EU/μg for transfection of highly sensitive cells
  • Patented endotoxin removal by additional washing step – no extra ice incubation necessary
  • Optimized column design – Midi prep in 45 min.
  • Column filter included – high filter flow-rates, parallel lysate clearing and loading onto the column
  • Improved silica material – typical yields of 400μg (Midi)
Applications
  • High-copy plasmids from E. coli
  • Low-copy plasmids from E. coli (increased buffer volumes required)

Specifications

Buffer Yes
Format Midi Gravity-flow Column
Isolation Technology Anion Exchange Chromatography Technology
No. of Reactions 10
RNase Yes
Sample Volume <200 mL (high copy), <400 mL (low copy)
Size <300 kbp
Yield 400μg

Safety and Handling

compliance-icons compliance-icons compliance-icons
Signal Word
  • Danger
 Hazard Category
  • Flammable liquid Category 3
  • Skin corrosion/irritation Category 2
  • Serious eye damage/eye irritation Category 2
  • Respiratory sensitiser Category 1
 Hazard Statement
  • H226-Flammable liquid and vapour.
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
 Precautionary Statement
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
 Supplemental information
  • MIXTURE LIST-Contains : 2-propanol/ethanol(diluted < 20 %)/sodium hydroxide solution(diluted < 2 %),dodecyl sulfate,sodium s
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