Macherey-Nagel™ NucleoSpin™ 96 Blood, 96-well kit for DNA from blood

Description

NucleoSpin™ 96 Blood is designed for the parallel isolation of genomic DNA from whole blood. After lysis of blood cells genomic DNA is selectively bound to a silica membrane while contaminants are washed away effectively. Elution is possible even under vacuum without cross-contamination. Due to its plate design and the possibility to perform the complete procedure under vacuum NucleoSpin™ 96 Blood can be easily adapted to common liquid handling instruments for fully automated processing without manual interactions. Kit components: NucleoSpin™ Blood binding plate, MN wash plate, buffers, proteinase K, 15mL tubes, cap strips, square-well block, rack with tube strips.

Required hardware:

Vacuum processing - the NucleoSpin 96 blood kits can be used manually with the NucleoVac 96 vacuum manifold. For vacuum processing of the 8-well strips the reusable starter set A, containing column holders A and dummy strips is required. For automation on laboratory platforms with standard 96-well vacuum chambers the use of the starter set A is also required.

• Silica membrane technology
• Yield: 4μg to 6μg
• Sample material: ≤200uL whole blood/2 x 10⁶ cultured cells
• Elution volume: 100μL
• Binding capacity: 20μg
• Preparation time: 70 min./plate
• Format: 96 well plates
• Fragment size: 300bp to 30kb
• No use of organic solvents Suitable for whole blood (human or animal); Whole blood treated with citrate, EDTA, heparin.