Macherey-Nagel™ NucleoSpin™ 96 Flash Filter Plates

Description

The NucleoSpin™ Multi-96 Plus Plasmid kit allows the fast purification of high copy-number plasmid DNA from E. coli in the 96 well format using a vacuum manifold or a benchtop centrifuge.
Sequencing grade plasmid DNA can be purified with a yield of up to 20μg from overnight cultures of up to 5mL LB, 3mL 2xYT, or 3mL TB. Using the NucleoSpin™ Multi-96 Plus Plasmid kit allows simultaneous processing of 96 samples within about 45min. The procedure is based on an alkaline lysis plasmid miniprep protocol. Bacteria are harvested by an initial centrifugation step. After resuspension of the pelleted bacteria and alkaline lysis the crude lysates are cleared by vacuum filtration with the NucleoSpin™ Plasmid filter plate. DNA is reversibly bound to the silice membrane of the NucleoSpin™ Plasmid Binding plate in a second filtration step. High purity of the final plasmid DNA preparation is achieved by removal of cellular compounds, salts, detergents, and other contaminants in subsequent washing steps. An additional washing step with buffer AW is recommended for E. coli host strains with high endogenous nuclease activity and for better performance of critical enzymatic reactions like long range DNA sequencing. Highly pure plasmid DNA is eluted with low-salt elution buffer (5mM Tris/HCl pH 8.5) or water (ph 8.0 to 8.5) and is ready to use for further applications.Kit components: NucleoSpin™ Plasmid filter plate, NucleoSpin™ Plasmid binding plate, square well block and cover sheet for bacteria cultivation, MN wash plate, elution plate including adhesive cover seal, buffers, RNase A.Nucleospin Multi-96 Plus Plasmid kit, isolation of plasmid DNA, buffer A1, A2, A3, AW, A4, AE, RNase A, Nucleospin filter plate and plasmid binding plate, MN wash plate, Elution plate including adhesive cover steel.For further information on this product contact Customer Services.

• Yield: 5μg to 15μg DNA/5mL overnight culture
• Elution volume: 75μL to 150μL
• Time/prep: 45min/1 x 96 well plate
• 96 well filter plates included for clarification of crude lysates
• Silica-membrane technology
• Culture volume: 1mL to 5mL
• Binding capacity: 20μg
• Maximum plasmid size <15kb
• No use of organic solvents

For manual 96 well small-scale purification of high copy plasmid DNA from E. coli in a microtitre plate format.