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  6. Macherey-Nagel™ NucleoSpin™ 96 Plasmid Transfection-grade Core Kit, 96-well kit

Macherey-Nagel™ NucleoSpin™ 96 Plasmid Transfection-grade Core Kit, 96-well kit

Product Code. 15553636
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No. of Samples:
24 x 96
4 x 96
Product Type:
Core Kit
Full Kit
Unit Size:
2304 reactions
384 reactions
This item is not returnable. View return policy

Product Code. 15553636

Brand: Macherey-Nagel™ 740492.4

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This item is not returnable. View return policy

Description

A fast way to purify transfection-grade plasmid DNA using mini-format technology to reduce endotoxins from bacterial lysates. Full and Core kits utilize a 96-well plate format for an efficient high-throughput process.

Endotoxins are co-purified during typical plasmid preparations from bacterial lysates. Since they interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection. NucleoSpin™ Plasmid Transfection-grade kits are designed to reduce endotoxins from bacterial lysates.

  • Purifies high-copy plasmids for transfection
  • Novel technology to diminish endotoxin content (< 50 EU/μg DNA) for successful transfection
  • Rapid purification from E. coli via automatic, manual, vacuum or centrifugation processing
  • Ultra-fast procedure simplifies the work-flow
  • High-throughput — 96-well plates allow for easy automation and supports plasmid screening
  • Downstream applications: transfection of common cell lines (lipofection, electroporation)
  • Also suitable for standard molecular biology application, such as cloning and sequencing

Specifications

Bacterial Endotoxins <50 EU/ng DNA
Content And Storage Room temperature (18 - 25 °C)
Format 96-well Plate
Isolation Technology Silica Membrane and Endotoxin-reduction Technology
Sample Type Bacterial Culture (E. coli, High-copy Plasmids)
Efficiency Binding capacity: 20 μg
Elution Volume Elution Volume: 100 - 200 μL
Final Product Type Transfection-grade plasmid DNA
For Use With (Equipment) Centrifuge, Vacuum manifold (e.g. NucleoVac 96)
For Use With (Application) Rapid purification of plasmids for transfection
Includes Filter Plates, Binding Plates, Buffers, RNase A
No. of Samples 4 x 96
Product Type Core Kit
Purity A260/A280: 1.80 - 1.85
Sample Volume Up to 5 mL
Size < 15 kbp
Test Time 45 min/plate
Yield 5 - 20 μg
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Safety and Handling

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Product Identifier
  • NucleoSpin 96 Plasmid T.-gr. Core (4x96)
 Signal Word
  • Danger
 Hazard Category
  • Acute toxicity Category 4
  • Serious eye damage/eye irritation Category 2
  • Flammable liquid Category 3
  • Respiratory sensitiser Category 1
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H226-Flammable liquid and vapour.
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H336-May cause drowsiness or dizziness.
 Precautionary Statement
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P264-Wash thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P330-Rinse mouth.
  • P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
 Supplemental information
  • MIXTURE LIST-Contains : sodium hydroxide, dodecyl sulphate, guanidine hydrochloride,triethanolamine, 2-propanol, RNAse
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