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Macherey-Nagel™ NucleoSpin™ miRNA Column / Buffer Set

Product Code. 15839067
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Includes 50 columns, 35 mL MW1, 20 mL MW2
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Product Code.
15839067 Includes 50 columns, 35 mL MW1, 20 mL MW2
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Product Code. 15839067 Supplier Macherey-Nagel™ Supplier No. 740304

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Description

Additional NucleoSpin RNA Columns and buffers for miRNA isolation with NucleoSpin miRNA kits.

  • Excellent total RNA recovery and purity by chaotropic salt lysis without phenol/chloroform
  • Support protocol for fractionation of RNA by size – small RNA (< 200 nt) and large RNA (> 200 nt) in two separate fractions
  • Additional isolation of total protein fraction ready to use for SDS-PAGE and Western blot analysis
  • NucleoSpin™ Filters for efficient sample homogenization
  • rDNase for efficient on-column removal of genomic DNA

Specifications

Format Mini Spin Column
Isolation Technology Silica Membrane
Elution Volume Elution volume: 30-100 μL
Sample Type <1 x 107 Cultured Cells, <30 mg Human/Animal Tissue, <50 mg Plant Tissue, <150 μL Reaction Mixture
Efficiency Binding capacity: 200 μg
Final Product Type small and large RNA
For Use With (Equipment) Centrifuge
For Use With (Application) Isolation of total RNA from diverse sample materials
Includes 50 columns, 35 mL MW1, 20 mL MW2 Concentrate
No. of Samples 50
Test Time < 45 min/6 preps (total RNA), < 35 min/6 preps (small RNA only)
Yield 100 μg total RNA (107 HeLa cells: 10 μg small RNA, 90 μg large RNA)
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Safety and Handling

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Product Identifier
  • NucleoSpin miRNA Column/Buffer Set
 Signal Word
  • Warning
 Hazard Category
  • Flammable liquid Category 3
 Hazard Statement
  • H226-Flammable liquid and vapour.
 Supplemental information
  • MIXTURE LIST-Contain: guanidium thiocyanide, ethanol
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