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Macherey-Nagel™ NucleoSpin™ RNA Blood Midi

Product Code. 15128584
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20 Preps
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15128584 20 Preps
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Product Code. 15128584 Supplier Macherey-Nagel™ Supplier No. 740210.20

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Description

Midi spin kit for superior isolation of total RNA from fresh or frozen whole blood (human or animal) from common blood collection tubes. Direct blood lysis process does not require the use of a selective erythrocyte lysis.

NucleoSpin™ RNA Blood Midi kit achieves superior RNA yield and quality in a format that provides convenient handling and flexible sample volume and throughput.

  • Direct total blood lysis—Very simple and convenient procedure
  • No selective erythrocyte lysis at 4 °C—Complete processing at room temperature
  • Superior RNA yield and quality from up to 1.3 mL whole blood
  • Efficient on-column DNA removal for an increased sensitivity in downstream applications
  • Compatible with common blood collection tubes and anticoagulants (e.g., EDTA, citrate, and heparin)

Specifications

Format Midi Spin Column
Isolation Technology Silica Membrane Technology
Elution Volume 200 to 400 μL
Sample Type Whole Blood (Fresh or Frozen)
Efficiency Binding capacity: 700 μg
Final Product Type Total RNA
For Use With (Equipment) Vortex Mixer, Centrifuge
For Use With (Application) RNA isolation
Includes NucleoSpin RNA Blood Midi Columns with Collection Tubes, Collection Tubes (15 mL), buffers, RNase-free rDNase, Liquid Proteinase K
No. of Reactions 20 Preps
Purity A260/A280: 1.9 - 2.1
Sample Size 400 to 1300 μL
Test Time 75 min/6 Preps
Yield 4 to 26 μL
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Safety and Handling

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Signal Word
  • Danger
 Hazard Category
  • Flammable liquid Category 3
  • Acute toxicity Category 4
  • Respiratory sensitiser Category 1
  • LONG-TERM AQUATIC HAZARD Chronic 3
 Hazard Statement
  • H226-Flammable liquid and vapour.
  • H302-Harmful if swallowed.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H412-Harmful to aquatic life with long lasting effects.
 Supplemental information
  • MIXTURE LIST-Contains : guanidinium thiocyanate; ethanol (diluted < 20 %) (denaturedwith 1%IPA/1%MEK, acc.2016/1867/EU); pr
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