missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Enzymes and Inhibitors
  4. Modifying Enzymes
  5. OPTIZYME™ Alkaline Phosphatase, Fisher BioReagents™

OPTIZYME™ Alkaline Phosphatase, Fisher BioReagents™

Product Code. 12897142
Change view
Click to view available options
Quantity:
3000 U
Unit Size:
3000 units
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Quantity unitSize
12897142 3000 U 3000 units
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
This item is not returnable. View return policy
Product Code. 12897142 Supplier Fisher BioReagents Supplier No. BP80975
Request Bulk or Custom Format

Please to purchase this item. Need a web account? Register with us today!

This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
 View alternative products

This item is not returnable. View return policy

Description

OPTIZYME™ AP is a novel alkaline phosphatase that catalyzes the release of 5'- and 3'-phosphate groups from DNA, RNA and nucleotides in 10min.

Applications and Features:

  • OPTIZYME™ Alkaline Phosphatase dephosphorylates cloning vector DNA (10min at 37°C)
  • Fast thermoinactivation (5min at 75°C)
  • Simultaneous digestion and dephosphorylation of vector DNA
  • Single protocol for all types of DNA ends
  • Nucleotide degradation prior to sequencing PCR products
  • Active on nucleotides
  • Protein dephosphorylation
  • Active in all OPTIZYME restriction enzyme buffers

Supplied with:
10X OPTIZYME AP Buffer: 100mM Tris-HCl (pH 8.0 at 37°C), 50mM MgCl2, 1M KCl, 0.2% (v/v) Triton X-100, 1mg/mL BSA
Source: E. Coli cells with a cloned bacterial alkaline phosphatase gene
Quality Control:

  • Endo-, exodeoxyribonuclease and ribonuclease-free
  • Functionally tested for dephosphorylation of 5'-termini of overhanging, recessed and blunt DNA

TRUSTED_SUSTAINABILITY

Specifications

Concentration 1 U/μL
Color Colorless
pH 7.4
For Use With (Application) DePhosphorylates cloning vector DNA (10 min. at 37°C), fast thermo-inactivation (5 min. at 75°C), Nucleotide degradation prior to sequencing PCR products, active on Nucleotides, Protein dephosphorylation, restriction En
Storage Buffer OPTIZYME™ AP Buffer 10X : Tris HCl 100mM (8 pH at 37°C), MgCl2 50mM, KCl 1M, Triton™ X-100 0.2%, BSA 1mg/mL
Content And Storage -20°C
Source E. coli cells with a cloned bacterial Alkaline Phosphatase gene
Quantity 3000 U
Product Type Alkaline Phosphatase
Form Solid
Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.