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OPTIZYME™ BglII, Fisher BioReagents™
270.00 € - 505.00 €
Specifications
Concentration | 10 U/μL |
---|---|
Components | 25 to 50% Water, >50% Glycerin, 1 to 2.5% Sodium Chloride |
Incubator Temperature | 37°C |
pH | 7.4 |
For Use With (Application) | >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with BglII |
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
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Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
11984852
|
Fisher Bioreagents
BP8014-5 |
2500 U |
505.00 €
Each |
Estimated Shipment: 19-06-2024 Log in to see stock. |
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11964852
|
Fisher Bioreagents
BP8014-1 |
1000 U |
This item is currently unavailable or has been discontinued. View the product page for possible alternatives. |
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Description
5'...A^G A T C T...3'
3'...T C T A G^A...5'
Supplied with: 10X OPTIZYME Buffer 3
Conditions for 100% Activity:
- 1X OPTIZYME Buffer 3: 50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 100mM NaCl and 0.1mg/ml BSA
- Incubate at 37°C
- Buffer 1: 0 - 20%
- Buffer 2: 20 - 50%
- Buffer 3: 100%
- Buffer 4: 0 - 20%
- Buffer 5: 50 - 100%
- 10mM Tris-HCl (pH 8.2 at 25°C), 200 mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/ml BSA and 50% (v/v) glycerol
- AGm6ATCT
- 5'...TGm6AGATCT(N)3 TGCT...3'
Specifications
10 U/μL | |
37°C | |
>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with BglII | |
Keep container tightly closed | |
BglII |
25 to 50% Water, >50% Glycerin, 1 to 2.5% Sodium Chloride | |
7.4 | |
10mM Tris HCl (pH 8.2 at 25°C), 200mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/mL BSA, 50% Glycerol | |
A.GATCT | |
Liquid |