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OPTIZYME™ DNase I (RNase-Free), Fisher BioReagents™

Product Code. 12887162
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Quantity:
1000 U
Unit Size:
1000 units
This item is not returnable. View return policy

Product Code. 12887162

Brand: Fisher BioReagents BP81071

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Description

OPTIZYME™ DNase I is a recombinant endonuclease that digests DNA and is RNase-Free.

Applications and Features:

  • OPTIZYME DNase I, RNase-Free is used for the preparation of DNA-free RNA
  • Removal of template DNA following in vitro transcription
  • DNA labeling by nick translation in conjunction with DNA Polymerase I
  • Preparation of DNA-free RNA prior to RT-PCR
  • Studies of DNA-protein interactions by DNase I, RNase-free footprinting
  • Enzyme activity is strictly dependent on Ca2+ and is activated by Mg2+or Mn2+ions
  • Purified from non-animal host with a lower level of intrinsic RNases

Supplied with:

  • 10X OPTIZYME DNase I Buffer: 100mM Tris-HCl (pH 7.5 at 25°C), 25mM MgCl2, 1mM CaCl2
  • 50mM OPTIZYME EDTA (for inactivation by heat at 65°C for 10min)
Source: E. Coli cells with a cloned gene encoding bovine DNase I
Quality Control:
  • Ribonuclease-free
  • Functionally tested for the digestion of template DNA after in vitro transcription

TRUSTED_SUSTAINABILITY

Specifications

Concentration 1 U/μL
Color Colorless
pH 7.5
For Use With (Application) Preparation of DNA-free RNA, removal of template DNA following in-vitro transcription, DNA labeling by nick translation in conjunction with DNA Polymerase I, Preparation of DNA-free RNA prior to RT-PCR, Studies of DNA-Protein interactions by DNase I, RNase-free footprinting
Storage Buffer OPTIZYME™ DNase I Buffer 10X : Tris HCl 100mM (7.5 pH at 25°C), MgCl2 25mM, CaCl2 1mM, OPTIZYME™ EDTA 50mM (for inactivation by heat at 65°C for 10min.)
Content And Storage -20°C
Source E. coli cells with a cloned gene encoding bovine DNase I
Quantity 1000 U
Product Type DNase I (RNase-free)
Form Liquid
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