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OPTIZYME™ M-MLV Reverse Transcriptase, Fisher BioReagents™

Product Code. 12837162
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Product Code. 12837162 Supplier Fisher BioReagents Supplier No. BP81045
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Description

OPTIZYME™ M-MuLV RT is a genetically modified MLV RT that possesses RNA- and DNA-dependent polymerase activity.

Applications and Features:

  • OPTIZYME M-MLV RT is used for efficient first strand cDNA synthesis for RT-PCR and real-time RT-PCR (up to 13kb)
  • Generation of labeled cDNA probes for microarrays
  • Synthesis of cDNA for cloning and expression
  • Analysis of RNA by primer extension
  • DNA labeling
  • Optimum activity at 42°C (active up to 50°C)
  • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, fluorescein-labeled nucleotides)

Supplied with:
5X OPTIZYME M-MLV RT Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 250mM KCl, 20mM MgCl2, 50mM DTT
Source: E. Coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus reverse transcriptase
Quality Control:

  • Endo-, exodeoxyribonuclease, phosphatase and ribonuclease-free
  • Functionally tested in first strand cDNA and RT-PCR

TRUSTED_SUSTAINABILITY

Specifications

Concentration 200 U/μL
Color Colorless
pH 8.3
For Use With (Application) Generation of labeled cDNA probes for microarrays, synthesis of cDNA for cloning and expression, analysis of RNA by primer extension, DNA labeling, optimum activity at 42°C, incorporates modified Nucleotides
Storage Buffer OPTIZYME™ M-MLV RT Buffer 5X : Tris HCl 250mM (8.3 pH at 25°C), KCl 250mM, MgCl2 20mM, DTT 50mM
Content And Storage -20°C
Source E. coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus Reverse Transcriptase
Quantity 25,000 U
Product Type OPTIZYME™ M-MLV Reverse Transcriptase
Form Liquid
Product Title
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