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OPTIZYME™ T4 DNA Polymerase, Fisher BioReagents™
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Quantity:
100 U
Packungsgröße:
100 units
Beschreibung
OPTIZYME™ T4 DNA Pol is a template-dependent DNA polymerase that catalyzes 5'->3' synthesis from primed single-stranded DNA.
Applications and features:
- OPTIZYME T4 DNA Polymerase creates blunt DNA ends by filling in 5'-overhangs and/or removing 3'-overhangs
- Creation of blunt PCR products with 3'-dA overhangs
- Oligonucleotide-directed site-specific mutagenesis
- Synthesis of labeled DNA probes (by replacement reaction)
- Ligation-independent cloning of PCR products
- Active in buffers for OPTIZYME restriction enzymes, reverse transcriptases and T4 DNA ligase
- 3'->5' exonuclease activity
- Lacks 5'->3' exonuclease activity
Supplied with:
5X OPTIZYME T4 DNA Pol Buffer: 335mM Tris-HCl (pH 8.8 at 25°C), 33mM MgCl2, 5mM DTT, 84mM (NH4)2SO4
Source: E. Coli cells with a cloned gene 43 of bacteriophage T4
Quality Control:
Endodeoxyribonuclease-free
Spezifikation
Spezifikation
| Concentration | 5 U/μL |
| Color | Colorless |
| pH | 7.5 |
| For Use With (Application) | Synthesis of labeled DNA probes, creation of blunt PCR products with 3'-dA overhangs, ligation-independent cloning of PCR products, Restriction Enzymes, reverse transcriptases and T4 DNA ligase |
| Storage Buffer | OPTIZYME™ T4 DNA Pol Buffer 5X: Tris HCl 335mM (8.8 pH at 25°C), MgCl2 33mM, DTT 5mM, (NH4)2SO4 84mM |
| Content And Storage | -20°C |
| Source | E. coli cells with a cloned gene 43 of bacteriophage T4 |
| Quantity | 100 U |
| Product Type | T4 DNA Polymerase |
| Form | Liquid |
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