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OPTIZYME™ T4 Polynucleotide Kinase, Fisher BioReagents™

Product Code. 12877142
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Quantity:
500 U
Unit Size:
500 units
This item is not returnable. View return policy

Product Code. 12877142

Brand: Fisher BioReagents BP80981

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Description

OPTIZYME™ T4 PNK catalyzes the transfer of γ-phosphate from ATP to the 5'-OH group of DNA, RNA, oligonucleotides and nucleoside 3'-monophosphates.

Applications and Features:

  • OPTIZYME™ T4 PNK labels 5'-termini of nucleic acids (for use as: probes for hybridization or transcript mapping, primers for PCR or DNA sequencing, and markers for gel-electrophoresis)
  • 5'-phosphorylation of oligonucleotides, PCR products, and other DNA or RNA prior to ligation
  • Phosphorylation of PCR primers
  • Removal of 3'-phosphate groups
  • Active in buffers for OPTIZYME restriction enzymes, T4 DNA Ligase and Reverse Transcriptases

Supplied With:

  • 10X OPTIZYME T4 PNK Buffer 1 (for forward reaction): 500mM Tris-HCl (pH7.6 at 25°C), 100mM MgCl2, 50mM DTT, 1mM spermidine
  • 10X OPTIZYME T4 PNK Buffer 2 (for exchange reaction): 500mM imidazole-HCl (pH6.4 at 25°C), 180mM MgCl2, 50mM DTT, 1mM spermidine, 1mM ADP
  • 24% PEG 6000 Sol for T4 PNK: 24% (w/v) polyethylene glycol 6000
Source: E. Coli cells with a cloned pse T gene of bacteriophage T4
Quality Control:
  • Endo-, exodeoxyribonuclease and ribonuclease-free
  • Functionally tested for labeling of 5'-termini of DNA

TRUSTED_SUSTAINABILITY

Specifications

Concentration 10 U/μL
Color Colorless
pH 7.5
For Use With (Application) Probes for hybridization or transcript mapping, primers for PCR or DNA sequencing and markers for Gel electrophoresis, removal of 3'-phosphate groups
Storage Buffer 10X OPTIZYME™ T4 PNK Buffer 1 (for forward reaction): 500mM Tris HCl (7.6 pH at 25°C), 100mM MgCl2, 50mM DTT, 1mM spermidine, 10X OPTIZYME™ T4 PNK Buffer 2 (for exchange reaction): 500mM imidazole-HCl
Content And Storage -20°C
Source E. coli cells with a cloned pse T gene of bacteriophage T4
Quantity 500 U
Product Type T4 Polynucleotide Kinase
Form Liquid
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