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Thermo Scientific™ Biocemical Identification System (O.B.I.S.) PYR

Product Code. 11483969
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60 Tests/Kit
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Product Code. 11483969 Supplier Thermo Scientific™ Supplier No. ID0580M

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Rapidly determine PYRase activity is streptococci and Citrobacter spp. with this biochemical identification system.

Rapidly determine PYRase activity is streptococci and Citrobacter spp. with Thermo Scientific™ Oxoid™ Biochemical Identification System (O.B.I.S.) PYR. This rapid colorimetric test delivers simple and rapid results for the determination of PYRase activity in streptococci and Citrobacter spp, offering a rapid diagnostic alternative to time-consuming culture methods. In addition, the same enzymatic activity may be used to aid differentiation of Salmonella spp. from Citrobacter spp and other Enterobacteriaceae1,2.

Use O.B.I.S. PYR as a rapid diagnostic alternative to time-consuming culture methods.

  • Simple—easy to implement
  • Rapid—results in 6 minutes of testing suspect colonies
  • Non-carcinogenic substrates

The O.B.I.S. - PYR test uses Test Cards impregnated with L-pyroglutamic acid 7-amino-4-methyl-coumarin (7AMC) and dimethylaminocinnamaldehyde for the detection of PYRase activity. The enzymatic hydrolysis of this substrate by enterococci, Group A streptococci and Citrobacter spp. produces a purple color following the addition of the Developing Solution3.

We have developed a new system for PYRase testing4 using a non-carcinogenic substrate in response to health concerns associated with the use of β-naphthylamide (a potent carcinogen)5. The PYRase area on the O.B.I.S. PYR test is impregnated with L-pyroglutamic acid 7-amino-4-methylcoumarin (7AMC)4. Dimethylaminocinnamaldehyde is used as a color development reagent. The enzymatic hydrolysis of the substrate produces a purple color on addition of the O.B.I.S. PYR Developing Solution4.

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.

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Specifications

Content And Storage 2°C to 8°C
Description O.B.I.S. PYR
Detectable Analytes PYRase activity
Includes Test cards, developing solution, buffer solution
Quantity 60 Tests/Kit
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