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Invitrogen™ Phosphate Sensor

Product Code. 10730434
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Quantity:
10 nmol
100 nmol; 10,000 assay(s)
Unit Size:
Each
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Product Code. Quantity unitSize
10730434 100 nmol; 10,000 assay(s) Each
10268302 10 nmol Each
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Product Code. 10730434 Supplier Invitrogen™ Supplier No. PV4407

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Description

Phosphate Sensor

The Phosphate Sensor is a highly sensitive reagent for the detection of inorganic phosphate, either in real-time kinetic or end-point mode. This product is a purified form of recombinant E. coli phosphate-binding protein labeled with the fluorophore MDCC, which is sensitive to changes in its environment. Binding of inorganic phosphate by Phosphate Sensor is rapid and tight (Kd ∼ 0.1μM), resulting in a large increase in fluorescence. The Phosphate Sensor detects phosphate in the high-nanomolar to low-micromolar range, which is 100-fold more sensitive than malachite green. This reagent provides you with a sensitive assay for detecting enzymes that produce inorganic phosphate either directly or indirectly through coupled reactions, such as phosphatases, ATPases, GTPases, and phosphodiesterases.
TRUSTED_SUSTAINABILITY

Specifications

For Use With (Equipment) Microplate Reader
Target GTPases, ATPases, Phosphatases, Phosphodiesterases
For Use With (Application) Fluorescence Intensity
Assay Phosphatase Assay
Content And Storage Phosphate Sensor is supplied as a 500 μM solution in 10 mM Tris (pH 7.6) and 50 mM NaCl. Store product at -80°C.
Detection Method Fluorescence
Label or Dye MDCC
Product Type Phosphate Sensor
Quantity 100 nmol; 10,000 assay(s)
Format 384-well plate
Excitation/Emission 430⁄450
Readout Kinetic
Shipping Condition Dry Ice
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For Research Use Only. Not for use in diagnostic procedures.

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