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Thermo Scientific™ Phosphorylating Phosphoramidite, standard grade, 20-400 finish bottle
Shop All Thermo Scientific ProductsBeschreibung
Phosphorylating phosphoramidite can be utilized to achieve phosphorylation of the 5' oligo terminus (also known as Phosphate-ON or Chemical Phosphorylation Reagent (CPR)). Chemical phosphorylation is a cost-effective substitute to enzymatic methods (via T4 kinase and ATP), allowing efficient introduction of terminal phosphate groups. Oligos with a 5'-phosphate are conducive to a number of various applications utilizing the ligation of oligos to each other, such as NGS adapters, cloning, and gene construction. This product is supplied in a 30 mL amber bottle with a 20-400 Finish neck style, which is compatible with most Expedite Systems of oligonucleotide synthesizers.
Features:
• Full chemical name: 2-[2-(4,4-Dimethoxytrityloxy)ethylsulfonyl]ethyl-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
• Molecular formula: C34H45F3N2O7PS
• Formula weight: 656.66 g/mol
• HPLC Purity: ≥95.0%
Spezifikation
Spezifikation
| For Use With (Equipment) | Expedite Synthesizers |
| Product Type | Phosphorylating CEP |
| Content And Storage | Store in freezer (at or below -20°C). |
| Nucleoside Type | None (Modifier) |
| Shipping Condition | Room Temperature |
| Purity | 95% |
| Quantity | 100 μmol |
| Reagent Type | Phosphoramidite |
| Grade | Standard |
| Nucleic Acid Type | None (Modifier) |
| Mehr anzeigen |
For Research Use Only. Not for use in diagnostic procedures.
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