Learn More
Thermo Scientific™ Phosphorylating Phosphoramidite, standard grade, 20-400 finish bottle
Shop All Thermo Scientific ProductsDescription
Phosphorylating phosphoramidite can be utilized to achieve phosphorylation of the 5' oligo terminus (also known as Phosphate-ON or Chemical Phosphorylation Reagent (CPR)). Chemical phosphorylation is a cost-effective substitute to enzymatic methods (via T4 kinase and ATP), allowing efficient introduction of terminal phosphate groups. Oligos with a 5'-phosphate are conducive to a number of various applications utilizing the ligation of oligos to each other, such as NGS adapters, cloning, and gene construction. This product is supplied in a 30 mL amber bottle with a 20-400 Finish neck style, which is compatible with most Expedite Systems of oligonucleotide synthesizers.
Features:
• Full chemical name: 2-[2-(4,4-Dimethoxytrityloxy)ethylsulfonyl]ethyl-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
• Molecular formula: C34H45F3N2O7PS
• Formula weight: 656.66 g/mol
• HPLC Purity: ≥95.0%
Specifications
Specifications
| For Use With (Equipment) | Expedite Synthesizers |
| Product Type | Phosphorylating CEP |
| Content And Storage | Store in freezer (at or below -20°C). |
| Nucleoside Type | None (Modifier) |
| Shipping Condition | Room Temperature |
| Purity | 95% |
| Quantity | 100 μmol |
| Reagent Type | Phosphoramidite |
| Grade | Standard |
| Nucleic Acid Type | None (Modifier) |
| Show More |
For Research Use Only. Not for use in diagnostic procedures.
By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.