missing translation for 'onlineSavingsMsg'
Läs mer
  1. Home
  2. Products
  3. PCR Equipment and Supplies
  4. PCR Supplies
  5. Standard PCR Products
  6. High Fidelity PCR Reagents and Kits
  7. Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases (2 U/μL)

Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases (2 U/μL)

Produktkod. p-4526976 Handla allt< titel> Produkter
missing translation for 'orderingAttributeHoverText'
Color:
Colorless
Green
No. of Reactions:
100 Reactions
2000 Reactions
500 Reactions
Förpackningsstorlek
100 units
2000 units
500 units
Denna artikel kan inte returneras. Se returpolicy

Produktkod. 10024537

missing translation for 'mfr': Thermo Scientific™ F530S

Please to purchase this item. Need a web account? Register with us today!

117.35 EUR valid until 2025-12-31
BEST PRICE on Promo! Use promo code "24159" to get your promotional price.
Denna artikel kan inte returneras. Se returpolicy

Beskrivning

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is an excellent choice for cloning and other applications requiring high fidelity. Phusion DNA polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerases.

Features of Phusion™ High-Fidelity DNA Polymerase include:

  • High fidelity (52X Taq)
  • Fast PCR due to short extension times (15–30 s/kb)
  • Robust performance, minimal optimization needed
  • High yields of PCR products with minimal enzyme amounts
  • Available in Green buffer format for direct loading of PCR products on gels (F-534S or F-534L)

Applications

  • High-fidelity PCR
  • Cloning
  • Template generation for sequencing
  • Amplification of difficult (GC-rich) templates
  • Long-range PCR (up to 20 kb)
  • Mutagenesis
  • High throughput PCR
  • Microarray

Note: Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our convenient Tm calculator.

TRUSTED_SUSTAINABILITY

Specifikationer

Concentration 2 U/μL
Content And Storage • Phusion DNA Polymerase (2 U/μL)
• 5X Phusion HF & GC Buffers
• DMSO
• 50 mM MgCl2 solution

Phusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl2 in the final 1X concentration.
Store at -20°C.
GC-Rich PCR Performance High
Polymerase Phusion High-Fidelity DNA Polymerase
Reaction Speed Fast
Product Type High-Fidelity DNA Polymerase
Quantity 100 units
Shipping Condition Dry Ice
For Use With (Application) Standard PCR, High-fidelity PCR
Fidelity (vs. Taq) 52X
Hot Start No
No. of Reactions 100 Reactions
Overhang Blunt
Reaction Format Standalone
Size (Final Product) 20 kb or less
Color Colorless
Visa mer Visa mindre

For Research Use Only. Not for use in diagnostic procedures.

Korrigering av produktinnehåll

Din input är viktig för oss. Fyll i det här formuläret för att ge feedback relaterad till innehållet på denna produkt.

Produkttitel

Genom att klicka på Skicka bekräftar du att du kan bli kontaktad av Fisher Scientific angående feedbacken du har lämnat i detta formulär. Vi kommer inte att dela din information för andra ändamål. All kontaktinformation som tillhandahålls ska också underhållas i enlighet med vår Sekretesspolicy.

Tack! Din feedback har skickats. Fisher Scientific arbetar alltid för att förbättra vårt innehåll åt dig. Vi uppskattar din feedback.