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Thermo Scientific™ Phusion Human Specimen Direct PCR Kit
Perform PCR directly from human samples including buccal swabs, saliva, hair, and fingernails without DNA purification.
Marke: Thermo Scientific™ F150BID
Code : NEW
Weitere Details : CAS Number : 7732-18-5 Weight : 2.28610kg
Beschreibung
Thermo Scientific Phusion Human Specimen Direct PCR Kit delivers unprecedented convenience for DNA amplification by allowing PCR directly from unpurified human samples. A tiny amount of sample is used directly in the PCR reaction with no prior purification steps. This allows significant savings in both time and cost.
Suitable sample materials include buccal swabs, saliva, amniotic fluid, hair, fingernails, teeth, and skin biopsies. The kit is compatible with fresh, frozen, and even Formalin-Fixed, Paraffin Embedded (FFPE) human tissue samples. It includes optimized reagents and detailed protocols for several sample materials. The modified Phusion Hot Start II High-Fidelity DNA Polymerase utilized in the kit is a highly accurate proofreading DNA polymerase; PCR products obtained are perfectly suitable even for cloning purposes. The kit is for research use only.
Highlights
- No need for time-consuming and expensive DNA purification steps
- Two simple protocols for various applications
- Extremely short PCR protocol times
- Modified Phusion Hot Start II High-Fidelity DNA Polymerase delivers abundant yields of specific product
Spezifikation
Contains: • 80 μL Phusion Human Specimen DNA Polymerase • 2 x 1.25 mL 2X Phusion Human Specimen PCR Buffer (includes dNTPs and MgCl2) • 40 μL Universal Control Primer Mix (25μM each) • 2 x 5 mL Dilution Buffer • 3 x 100 μL DNARelease Additive Store at -20°C. |
|
Direct PCR | |
Fast | |
Direct PCR Kit | |
200 Reactions | |
Separate Components | |
DNA |
High | |
Phusion Human Specimen DNA Polymerase | |
Phusion | |
Built-In Hot Start | |
Blunt | |
Human |
For Research Use Only. Not for use in diagnostic procedures.