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Descrizione
Thermo Scientific Phusion U DNA Polymerase is a novel engineered high fidelity enzyme developed using fusion technology. Due to a proprietary mutation in the so called dUTP binding pocket of Phusion polymerase, Phusion U polymerase overcomes an important limitation of proofreading enzymes: it is able to incorporate dUTP and read through uracil present in DNA templates.
In addition to the uracil usage possibility, Phusion U polymerase features all the superior properties of other Phusion DNA polymerases: great accuracy, speed, ability to amplify long amplicons up to 20 kb, and a high specificity with Affibody-based hot start. These features make Phusion U Hot Start DNA Polymerase an excellent choice for such important applications as amplification of bisulphite-converted or damaged DNA as well as use of carryover contamination control.
Phusion U Hot Start Green DNA Polymerase is a combination of Phusion U Hot Start DNA Polymerase and 5X Phusion Green buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with Phusion U polymerase performance and is compatible with downstream applications such as DNA sequencing, ligation, and restriction digestion.
Highlights
- Accuracy—high fidelity DNA polymerase (25X Taq)
- Uracil-tolerance—engineered to incorporate dUTP and amplify uracil containing templates
- Specificity—hot start for reduced nonspecific amplification and primer degradation
- Speed—short extension times (15–30 s/kb)
- Green format—permits direct loading of PCR products on gels
Applications
- Amplification of bisulphite-converted DNA
- Amplification of damaged or aged DNA
- Carry-over contamination control
- Uracil-excision based (USER) cloning methods
Using Phusion DNA polymerases
Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).
Specifica
Specifica
| Concentration | 2 U/μL |
| Content And Storage | • Phusion U Hot Start DNA Polymerase, 2 U/μL • 5X Phusion Green HF & GC Buffers • DMSO • 50 mM MgCl2 solution Phusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl2 in the final 1X concentration. Store at -20°C. |
| GC-Rich PCR Performance | High |
| Polymerase | Phusion U Hot Start DNA Polymerase |
| Reaction Speed | Fast |
| Product Type | Hot Start DNA Polymerase |
| Quantity | 500 units |
| Shipping Condition | Dry Ice |
| For Use With (Application) | Hot-start PCR, High-fidelity PCR |
| Fidelity (vs. Taq) | 25X |
| Vedi altri risultati |
For Research Use Only. Not for use in diagnostic procedures.
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