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Descrizione
Thermo Scientific Pierce Anti-DYKDDDDK Magnetic Agarose provides a fast, convenient method for purification and immunoprecipitation (IP) of DYKDDDDK-tagged proteins from in vitro protein expression systems, bacteria, yeast, and mammalian cells. The amino acid sequence DYKDDDDK, commonly known as 'FLAG', is recognized by a high-affinity rat monoclonal antibody (clone L5) that is covalently attached to a magnetite-embedded agarose core particle.
For protein purification, the magnetic agarose is added to a sample containing DYKDDDDK-tagged proteins with the tag on either the N- or the C-terminus. Captured proteins are then magnetically separated from the supernatant, and non-specifically bound proteins can be washed away before dissociating bound DYKDDDDK-tagged proteins with elution buffer. The magnetic agarose is removed from the solution using a magnetic stand or an instrument such as the KingFisher Flex Magnetic Particle Processor. Automated instruments are especially useful for higher throughput purifications and screening of purification conditions.
Features include:
- Specific—unique base beads and highly specific antibody minimizes off-target binding (low non-specific binding)
- High purity—optimized bind-wash-elute protocol enables high purity
- High yield—special antibody conjugation method enables high yield
- Rapid—entire purification protocol typically takes less than 40 mins
- Economical—purification protocol allows multiple reuses
- Versatile—beads are compatible with manual and automated workflows (e.g., KingFisher instruments)
Characteristics of Pierce Anti-DYKDDDDK Magnetic Agarose:
- Composition: anti-DYKDDDDK antibody covalently attached to a magnetic, highly crosslinked agarose support
- Magnetization: ferrimagnetic with low remanence
- Bead size: 10–40 μm
- Bead concentration: 25% slurry in phosphate buffered saline, 0.01% Tween-20 detergent, 0.02% sodium azide, pH 7.2
- Binding capacity: ≥3.2 mg DYKDDDDK-tGFP-His protein (∼32 kDa)/mL settled beads
Specifica
Specifica
| Product Line | Pierce |
| Product Type | Magnetic Agarose |
| Bed Volume | 1 mL |
| Quantity | 4 mL |
| Particle Size | 25 to 30 μm |
| Formulation | Slurry: 25% |
| Content And Storage | 1 mL settled beads. 4 mL supplied as a 25% suspension in PBS containing 0.02% sodium azide. Store at 2–8°C. |
| Capacity | 4 mL |
| Format | Liquid |
| Stationary Phase | Anti-DYKDDDDK monoclonal antibodies |
| Vedi altri risultati |
For Research Use Only. Not for use in diagnostic procedures.
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