Learn More
Thermo Scientific™ Pierce™ Microplate BCA Protein Assay Kit - Reducing Agent Compatible
Shop All Thermo Scientific ProductsDescription
This reducing agent-compatible (RAC) version of the Thermo Scientific™ Pierce BCA Protein Assay provides accurate determination of protein concentration in samples that contain dithiothreitol (DTT), 2-mercaptoethanol (BME), TCEP and other disulfide reducing agents.
Highlights:
Compatible – assay samples that contain up to 5mM DTT, 35mM BME, or 10mM TCEP
BCA technology – only a slight modification of the standard BCA Protein Assay protocol (15-minute incubation with Compatibility Reagent); no precipitation steps required
Small samples – requires only 25μL (standard kit) or less than 10μL (microplate kit) of sample
Colorimetric – measure with a standard spectrophotometer or plate reader (562nm)
Excellent uniformity – exhibits less protein-to-protein variation than dye-binding methods
High linearity – linear working range for BSA equals 125 to 2000μg/mL
Includes:
BCA Reagents A and B, Compatibility Reagent, Reconstitution Buffer, and Albumin Standard (2mg/mL); Microplate kit also includes assay-compatible 96-well microplates
Spécification
Spécification
| Content And Storage | Sufficient For: 980 microplate assays with 9 μL samples • BCA Reagent A, 250 mL • BCA Reagent B, 25 mL • Compatibility Reagent, 48 x 10 mg • Reconstitution Buffer, 15 mL • Albumin Standard Ampules, 2mg/mL, 10 x 1 mL • 96-Well Microplates, 20 plates Store at room temperature |
| Description | Pierce BCA Microplate Protein Assay Kit, Reducing Agent Compatible (RAC) |
| Assay | BCA Assay |
| Detection Method | Colorimetric |
| Product Line | Pierce |
| Product Type | Protein Quantitation Assay |
| Specificity | Not Target-Specific |
| Sufficient For | 980 Microplate Assays |
| For Use With (Application) | Solution-based Detection, Absorbance |
| For Use With (Equipment) | Spectrophotometer, Microplate Reader |
| Afficher plus |
Sécurité et traitement
- Pierce Microplate BCA Protein Assay Kit - Reducing Agent Compatible
- Danger
- Acute toxicity Category 3
- Respiratory sensitiser Category 1
- Skin sensitiser Category 1
- ACUTE AQUATIC Acute 1
- LONG-TERM AQUATIC HAZARD Chronic 2
- H301-Toxic if swallowed.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- H317-May cause an allergic skin reaction.
- H400-Very toxic to aquatic life.
- H411-Toxic to aquatic life with long lasting effects.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P284-¢In case of inadequate ventilation! wear respiratory protection.
- P273-Avoid release to the environment.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P391-Collect spillage.
- P302+P352-IF ON SKIN: Wash with plenty of water/.
- P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P405-Store locked up.
- P501-Dispose of contents/container to .
- MIXTURE LIST-Contains : sodium carbonate, ¢2, 2'-Biquinoline!-4,4'-dicarboxylic acid, sodium salt (1:2), Sulfuric acid copp
For Research Use Only. Not for use in diagnostic procedures.
Veuillez fournir vos retours sur le contenu du produit en remplissant le formulaire ci-dessous.