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  5. Thermo Scientific™ Pierce™ C18 Spin Tips & Columns

Thermo Scientific™ Pierce™ C18 Spin Tips & Columns

Product Code. 10615555 Shop All Thermo Scientific Products
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Quantity:
25 Columns
50 Columns
96 Tips
96 x 10 μL Tips
96 x 100 μL Tips
Product Type:
Column
Pipette Tip
Spin Tip
Unit Size:
Pack of 25
Pack of 50
Pack of 96
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Product Code. Quantity Product Type unitSize
10615555 96 x 100 μL Tips Pipette Tip Pack of 96
11814141 25 Columns Column Pack of 25
11824141 50 Columns Column Pack of 50
13404219 96 Tips Spin Tip Pack of 96
10627275 96 x 10 μL Tips Pipette Tip Pack of 96
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Product Code. 10615555 Supplier Thermo Scientific™ Supplier No. 87784
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352.35 EUR valid until 2026-07-03
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Description

Thermo Scientific Pierce C18 Spin Columns (10–150 μL) and Spin Tips (10–100 μL) effectively remove interfering contaminants, while also maintaining excellent binding and recovery of peptide samples for use upstream of mass spectrometry and other high sensitivity detection methods.

C18 Spin Tips for Peptide Desalting and Cleanup

Pierce™ C18 spin tips are designed for rapid, reproducible peptide desalting, cleanup, and concentration prior to mass spectrometry analysis. Utilizing reversed-phase C18 chemistry, these tips help enable efficient peptide binding and recovery while removing salts, detergents, and other contaminants that can interfere with downstream applications.

Used in combination with Pierce Spin Tip Adaptors (Catalog No. A40011776) and low protein binding microcentrifuge tubes (Catalog No. 90410), C18 spin tips support a simple centrifugation-based workflow that delivers consistent and reliable results across a wide range of sample inputs.

Features of C18 Spin Tips

  • Reliable – proven reversed-phase C18 chemistry
  • Efficient – effectively removes interfering contaminants to improve spectral quality
  • Streamlined – simple workflow using tip adaptor and centrifugation steps

Applications

  • Peptide desalting prior to LC-MS analysis
  • Cleanup following protein digestion workflows
  • Removal of salts, buffers, and contaminants
  • Peptide concentration and sample preparation

How to Use

C18 spin tips use a bind–wash–elute workflow performed by centrifugation. Tips are placed into a tip adaptor seated in a microcentrifuge tube for each step. Simply activate and equilibrate prior to adding your acidified sample. To enhance peptide binding repeat the sample loading step one time. Lastly, wash 2-3 times and elute into a fresh tube using an acetonitrile solution. Refer to Table 1 in the Figures for recommended buffers, volumes and spin speeds for each tip size.

TRUSTED_SUSTAINABILITY

Specifications

Bed Volume 100 μL
Description Pierce C-18 Tips, 100 μL bed
For Use With (Equipment) Microcentrifuge, Mass Spectrometer
Product Type Pipette Tip
Product Line Pierce
Content And Storage Store at room temperature.
Starting Material Peptides, Protease-digested Protein
Final Product Type Peptides
Detection Method Mass Spectrometry
Format Tip
Workflow Step Sample Clean Up
Quantity 96 x 100 μL Tips
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