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Description
C18 Spin Tips for Peptide Desalting and Cleanup
Pierce™ C18 spin tips are designed for rapid, reproducible peptide desalting, cleanup, and concentration prior to mass spectrometry analysis. Utilizing reversed-phase C18 chemistry, these tips help enable efficient peptide binding and recovery while removing salts, detergents, and other contaminants that can interfere with downstream applications.
Used in combination with Pierce Spin Tip Adaptors (Catalog No. A40011776) and low protein binding microcentrifuge tubes (Catalog No. 90410), C18 spin tips support a simple centrifugation-based workflow that delivers consistent and reliable results across a wide range of sample inputs.
Features of C18 Spin Tips
- Reliable – proven reversed-phase C18 chemistry
- Efficient – effectively removes interfering contaminants to improve spectral quality
- Streamlined – simple workflow using tip adaptor and centrifugation steps
Applications
- Peptide desalting prior to LC-MS analysis
- Cleanup following protein digestion workflows
- Removal of salts, buffers, and contaminants
- Peptide concentration and sample preparation
How to Use
C18 spin tips use a bind–wash–elute workflow performed by centrifugation. Tips are placed into a tip adaptor seated in a microcentrifuge tube for each step. Simply activate and equilibrate prior to adding your acidified sample. To enhance peptide binding repeat the sample loading step one time. Lastly, wash 2-3 times and elute into a fresh tube using an acetonitrile solution. Refer to Table 1 in the Figures for recommended buffers, volumes and spin speeds for each tip size.
Specifications
Specifications
| For Use With (Equipment) | Microcentrifuge, Mass Spectrometer |
| Product Type | Column |
| Product Line | Pierce |
| Content And Storage | Store at room temperature. |
| Starting Material | Peptides, Protease-digested Protein |
| Final Product Type | Peptides |
| Detection Method | Mass Spectrometry |
| Workflow Step | Sample Clean Up |
| Quantity | 25 Columns |
Frequently Asked Questions (FAQs)
Yes. C18 clean-up is necessary to remove small molecules that may interfere with phosphopeptide enrichment. Drying after clean-up is also necessary to concentrate the dilute samples and remove C18 organic eluting solvent so samples can be dissolved in the optimized buffers for phosphopeptide binding. Alternative binding buffers are not recommended as phosphopeptide yield and specificity will be affected. For maximal performance, ensure that the clean, dried peptide samples are completely solubilized in binding buffer with agitation for at least 10 min.
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