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Thermo Scientific™ Pierce™ CN/DAB Substrate Kit

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Quantity:
275 mL
Unit Size:
1 set
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Product Code. 10322284

Brand: Thermo Scientific™ 34000

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173.85 EUR valid until 2025-12-31
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This item is not returnable. View return policy

Description

Combines chloronaphthol- and diaminobenzidine-based HRP detection for Western blot and tissue staining methods.

Thermo Scientific™ Pierce CN/DAB Substrate Kit includes substrate and peroxide solutions for combined chloronaphthol- and diaminobenzidine-based detection of horseradish peroxidase (HRP) activity in blotting and tissue staining methods.

This kit combines two popular chromogenic peroxidase substrate compounds (4-CN and DAB) in a single, stable, 10X stock solution. This CN/DAB solution produces an intense dark black precipitate at sites of bound HRP-conjugated antibodies on probed blots and tissue samples. The Pierce CN/DAB Substrate Kit can be used in Western immunoblotting techniques as well as in dot blotting assays. The kit includes the 10X solution of CN/DAB and accompanying 1X Stable Peroxide Substrate Buffer.

Highlights:

  • HRP substrate – for detection of horseradish peroxidase activity on solid media via the combined action of CN (4-chloro-1-naphthol) and DAB (3,3'-diaminobenzidine tetrahydrochloride)

  • Chromogenic – no special equipment needed for visualization; yields intense black bands that are easy to photograph

  • Easy-to-use kit – substrate stock solution and stable peroxide substrate buffer mean that there is no dry powders to measure and dissolve

  • Stable – store refrigerated at 4°C for at least one year

Includes:

10X solution of CN/DAB and the Pierce Stable Peroxide Substrate Buffer

TRUSTED_SUSTAINABILITY

Specifications

Product Type Substrate
Detection Method Colorimetric
Form Liquid
Product Line Pierce™
Quantity 275 mL
Conjugate HRP
Substrate Horseradish Peroxidase
Reactivity Horseradish Peroxidase
Sufficient For 25 Uses at 10 mL Each
Content And Storage Sufficient For: 25 uses at 10 mL each
• CN/DAB (10X), 25 mL
• Stable Peroxide Buffer, 250 mL

Store at 4°C.

Safety and Handling

compliance-icons compliance-icons compliance-icons
Product Identifier
  • CN/DAB Substrate Kit
 Signal Word
  • Danger
 Hazard Category
  • Acute toxicity Category 3
  • Flammable liquid Category 2
  • Specific target organ toxicity after single exposure Category 1
 Hazard Statement
  • H225-Highly flammable liquid and vapour.
  • H301-Toxic if swallowed.
  • H311-Toxic in contact with skin.
  • H331-Toxic if inhaled.
  • H370-Causes damage to organs.
 Precautionary Statement
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : methanol

For Research Use Only. Not for use in diagnostic procedures.

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