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Thermo Scientific™ Pierce™ CN/DAB Substrate Kit

Product Code. 10322284 Shop All Thermo Scientific Products
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Quantity:
275 mL
Unit Size:
1 set
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10322284 275 mL 1 set
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Product Code. 10322284 Supplier Thermo Scientific™ Supplier No. 34000

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Description

Combines chloronaphthol- and diaminobenzidine-based HRP detection for Western blot and tissue staining methods.

Thermo Scientific™ Pierce CN/DAB Substrate Kit includes substrate and peroxide solutions for combined chloronaphthol- and diaminobenzidine-based detection of horseradish peroxidase (HRP) activity in blotting and tissue staining methods.

This kit combines two popular chromogenic peroxidase substrate compounds (4-CN and DAB) in a single, stable, 10X stock solution. This CN/DAB solution produces an intense dark black precipitate at sites of bound HRP-conjugated antibodies on probed blots and tissue samples. The Pierce CN/DAB Substrate Kit can be used in Western immunoblotting techniques as well as in dot blotting assays. The kit includes the 10X solution of CN/DAB and accompanying 1X Stable Peroxide Substrate Buffer.

Highlights:

  • HRP substrate – for detection of horseradish peroxidase activity on solid media via the combined action of CN (4-chloro-1-naphthol) and DAB (3,3'-diaminobenzidine tetrahydrochloride)

  • Chromogenic – no special equipment needed for visualization; yields intense black bands that are easy to photograph

  • Easy-to-use kit – substrate stock solution and stable peroxide substrate buffer mean that there is no dry powders to measure and dissolve

  • Stable – store refrigerated at 4°C for at least one year

Includes:

10X solution of CN/DAB and the Pierce Stable Peroxide Substrate Buffer

TRUSTED_SUSTAINABILITY

Specifications

Product Type Substrate
Detection Method Colorimetric
Form Liquid
Product Line Pierce
Quantity 275 mL
Conjugate HRP
Substrate Horseradish Peroxidase
Reactivity Horseradish Peroxidase
Sufficient For 25 Uses at 10 mL Each
Content And Storage Sufficient For: 25 uses at 10 mL each
• CN/DAB (10X), 25 mL
• Stable Peroxide Buffer, 250 mL

Store at 4°C.

Safety and Handling

compliance-icons compliance-icons compliance-icons
Product Identifier
  • CN/DAB Substrate Kit
 Signal Word
  • Danger
 Hazard Category
  • Acute toxicity Category 3
  • Flammable liquid Category 2
  • Specific target organ toxicity after single exposure Category 1
 Hazard Statement
  • H225-Highly flammable liquid and vapour.
  • H301-Toxic if swallowed.
  • H311-Toxic in contact with skin.
  • H331-Toxic if inhaled.
  • H370-Causes damage to organs.
 Precautionary Statement
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contain: methanol

For Research Use Only. Not for use in diagnostic procedures.

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