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Thermo Scientific™ Pierce™ Dilution-Free™ BSA Protein Standards, multichannel pipette compatible
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Descrizione
Pierce Dilution-Free BSA Protein Standards are high-quality bovine serum albumin reference samples for generating accurate standard curves and calibration controls in total protein assays. They are packaged as a set of seven pre-diluted standards in a multichannel pipette-friendly tubestrip, eliminating the need for serial dilutions. The tubestrip includes a single empty tube that enables users to add their own sample buffer for the purpose of blank subtraction.
Features of Dilution-Free BSA Protein Standards include:
• Multichannel pipette compatible—unique tubestrip design allows simultaneous transfer of a full standard curve dilution series to a microplate
• Convenient—complete set containing seven ready-to-use dilutions
• Universal—recognized as the industry standard for protein quantitation in colorimetric protein assays
• Pure and stable—supplied in ultrapure 0.9% saline solution with 0.05% sodium azide
• Accurate—reduce pipetting error from serial dilutions
These bovine serum albumin (BSA) solutions are protein concentration reference standards for use in BCA, Bradford, and other protein assay protocols. BSA is the universally accepted reference protein for total protein quantitation. The set of standards is prepared from a stock solution that is calibrated by direct comparison to purified BSA (Fraction V) from the National Institute of Standards and Technology (NIST).
Applications include:
• Protein assay quantitation standard (BCA Protein Assay, Bradford Assay, etc.)
• Protein recovery control for desalting and other column procedures
• General calibration of spectrophotometer UV-lamp (absorbance at 280 nm)
Selection of a protein standard is potentially the greatest source of error in any protein assay. The best choice for a standard is a purified, known concentration of the most abundant protein contained in the samples being tested. Often, a highly purified, known concentration of the protein of interest is not available or is too expensive to use as the standard or the sample itself is a mixture of many proteins (e.g., cell lysate). In such cases, the best standard is one that will produce a normal (i.e., average) color response curve with the selected protein assay method and is readily available to any researcher. BSA is such a protein, and the Pierce Dilution-Free BSA Protein Standards are the most convenient source of ready-to-use BSA standard.
For greatest accuracy in estimating total protein concentration in unknown samples, it is essential to include a standard curve each time the assay is performed. This is particularly true for the protein assay methods that produce non-linear standard curves. Deciding on the number of standards and replicates used to define the standard curve depends upon the degree of non-linearity in the standard curve and the degree of accuracy required. In general, fewer points are needed to construct a standard curve if the color response is linear. Typically, standard curves are constructed using at least two replicates for each point on the curve.
Specifica
Specifica
| Concentration | BSA: 0.125, 0.25, 0.5, 0.75, 1, 1.5, 2 mg/mL |
| Content And Storage | BSA in 0.9% sodium chloride with 0.05% sodium azide Store at 2°C to 8°C after opening. |
| Assay | Protein Standards |
| Product Line | Pierce, Dilution-Free |
| Product Type | Protein Quantitation Assay |
| Specificity | Not Target-specific |
| For Use With (Application) | Internal Standard |
| Quantity | 8-channel tubestrip (2 x 1 mL) |
Frequently Asked Questions (FAQs)
Yes, we offer the Pierce Dilution-Free BSA Protein Standards, Multichannel Pipette Compatible in two versions:
- Cat. No. A56979 (0.125-10 mg/mL) for the Pierce Dilution-Free Rapid Gold BCA Protein Assay Kit (Cat. No. A55860, A55861, A55862)
- Cat. No. A55863 (0.125-2 mg/mL) which is compatible with the Pierce BCA Protein Assay Kit (Cat. No. A55864, A55865) and with most protein assays that have a working range between 0-2 mg/mL
The Pierce Dilution-Free BSA Protein Standards, Multichannel Pipette Compatible is dissolved in 0.9% saline (sodium chloride) containing 0.05% sodium azide.
The BSA in the Pierce Dilution-Free BSA Protein Standards, Multichannel Pipette Compatible (Cat. No. A55863 and A56979) is identical to the BSA found in Pierce Bovine Serum Albumin Standard Ampules, 2 mg/mL (Cat. No. 23209) and the Pierce Bovine Serum Albumin Standard Pre-Diluted Set (Cat. No. 23208).
Pierce Dilution-Free BSA Protein Standards are provided in a convenient 8-channel tubestrip. Each tube contains 1 mL of pre-diluted BSA protein standard at each concentration required for generating a standard curve.
- Pierce Dilution-Free BSA Protein Standards, Multichannel Pipette Compatible, 0.125-2 mg/mL (Cat. No. A55863) includes BSA concentrations of 2000, 1500, 1000, 750, 500, 250, and 125 µg/mL.
- Pierce Dilution-Free BSA Protein Standards, Multichannel Pipette Compatible, 0.125-10 mg/mL (Cat. No. A56979) contains BSA concentrations of 10000, 5000, 2000, 1000, 500, 250, and 125 µg/mL.
Each tubestrip contains one empty tube which allows the user to add their own blank solution for the purpose of background correction. This convenient format eliminates the time-consuming dilution step required for other single-concentration protein standard formats like the Pierce Bovine Serum Albumin Standard Ampules, 2 mg/mL (Cat. No. 23209) or Pierce Bovine Serum Albumin Standard, 2 mg/mL (Cat. No. 23210).
Additionally, the tubestrips are compatible with multichannel pipettes which allows the user to easily transfer the full standard curve dilution series (including blank) to a microplate simultaneously. This minimizes setup time associated with traditional pre-diluted protein standards which are typically packaged in 7 separate vials, requiring the user to open each vial and pipette each standard individually.
We offer BSA protein standards at a concentration of 2 mg/mL in two different pack sizes:
10 x 1 mL in sealed glass ampules (Cat. No. 23209)
50 mL in a plastic bottle (Cat. No. 23210)
We also offer a Pre-Diluted Set of BSA standards in individual plastic vials which contain 3.5 mL of BSA protein standard per vial (Cat. No. 23208) with BSA concentrations of 2000, 1500, 1000, 750, 500, 250, and 125 µg/mL.
For Research Use Only.
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