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Thermo Scientific™ Pierce™ NHS-Fluorescein Antibody Labeling Kit

Product Code. 10628664 Shop All Thermo Scientific Products
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3 reactions kit
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Product Code. 10628664 Supplier Thermo Scientific™ Supplier No. 53029
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Estimated Shipment: 25-09-2026
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Description

Activated derivatives of green-fluorescent fluorescein dye to label antibodies, proteins and other molecules for fluorescence imaging (ex 494; em 518).

Thermo Scientific™ FITC and Fluorescein Labeling Reagents and Kits are high-performance derivatives of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.

Fluorescein dyes are used in wide-ranging applications including fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. FITC and NHS-fluorescein are reactive toward primary amine groups on proteins, peptides and other biomolecules, while fluorescein-5-maleimide and 5-IAF react with free sulfhydryls on cysteine residues.Most activated fluorescein derivatives are mixture of isomers with reactive groups attached at the 5- and 6-positions of the bottom ring. The properties of these isomers are indistinguishable in terms of excitation and emission spectra and for protein applications there is no need to isolate a specific isomer. The single isomer is used in high resolution separation techniques such as sequencing and capillary electrophoresis.

Highlights:

  • Amine-specific labeling: the NHS-ester or isothiocyanate efficiently labels antibodies and other purified proteins at primary amines (lysine side chains) Optimized procedure: following the standard protocol results in antibodies with excellent dye:protein ratios for optimum activity and fluorescence Single-use fluors: no need to weigh tiny amounts powder; kits contain single-use vials of reagent Efficient purification: kits include purification resin and easy-to-use spin columns, ensuring rapid and efficient removal of non-reacted dye and excellent protein recovery
TRUSTED_SUSTAINABILITY

Specifications

Description Pierce Fluorescein Antibody Labeling Kit
Product Type Antibody Labeling Kit
Quantity 3 reactions kit
Sufficient For Labeling and purifying 3 x 1 mg (2 mg/mL) of IgG or other proteins
Content And Storage Sufficient For: Labeling and purifying 3 x 1 mg (2 mg/mL) of IgG or other proteins
  • NHS Fluorescein, 3 x 50 μg vials
  • Borate Buffer, 1 mL
  • Purification Resin, 5 mL
  • Spin Columns, 6 each
  • Collection Tubes, 12 each

    Store NHS-Fluorescein at -20°C. Store other kit components at 4°C.
    		•
    Excitation/Emission 494/518
    Labeling Scale 0.5 to 1 mg
    Product Line Pierce
    Solubility DMF (Dimethylformamide), DMSO (Dimethylsulfoxide)
    Label or Dye FITC (Fluorescein)
    Labeling Target Antibodies, Proteins
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    For Research Use Only. Not for use in diagnostic procedures.

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