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Description
Assay regulatory element transcriptional activity in mammalian culture media or whole cell lysates with this highly sensitive flash-based Gaussia luciferase kit.
The Thermo Scientific Pierce Gaussia Luciferase Flash Assay Kit provides researchers with the most sensitive assay for transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysate.
Features of the Gaussia Luciferase Flash Assay Kit:
- Sensitive—1000 times greater sensitivity allows utilization of smaller numbers of cells
- Cost effective—highest sensitivity assays result in decreased reagent consumption
- Secreted luciferase—allows real-time assays and kinetic studies without destroying cells
- Time-saving—assays using secreted luciferases require minimal sample handling
- Automation-friendly—amenable to high throughput screens
- Convenient—contains a universal cell lysis buffer and optimized flash assay reagent
- Safe—allows one to perform non-radioactive assays
This Flash Assay Kit contains reagents for measuring the activity of Gaussia luciferase in mammalian cell culture media and lysates. When used with Thermo Scientific Gaussia Luc Vectors, the kit provides an extremely sensitive bioluminescent reporter assay system for secreted or intracellular detection of promoter or pathway activity. Gaussia luciferase is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity. The signal produced by Gaussia is considerably greater than signal from either firefly or Renilla luciferases assayed under similar conditions.
Includes:
Cell lysis buffer, reaction buffer and substrate
Requires:
Gaussia luciferase and luminometer or other instrument capable of monitoring luminescence, such as the Varioskan ALF or the Varioskan LUX microplate readers.
Applications:
- Promoter studies for analyzing cis- regulatory elements and trans-acting factors
- Drug screening
- siRNA and miRNA screening
- Multiplexed assays to study off-target effects
- Secretory pathway/protein localization reporter assays
- Signal transduction pathway analysis
- RNA splicing studies
Gaussia luciferase is a 20kDa protein from the marine copepod, Gaussia princeps. The bioluminescent enzyme is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity. Light output generated by the luciferase reaction can be correlated to the amount of Gaussia luciferase protein produced and used to determine the activity of the promoter driving Gaussia expression. The signal produced by Gaussia luciferase shows strong flash kinetics and is considerably greater than flash signals from either firefly or Renilla luciferases assayed under similar conditions. The assay kit is optimized for use with our Gaussia Luc Vectors but is fully compatible with other Gaussia luciferases and derivatives that use coelenterazine as a substrate. (For glow assays, use our Gaussia-Dura Luc Vectors.)
Specifications
Specifications
| Type | Assay Kit |
| Target | Luciferase, Gaussia Luciferase |
| Sufficient For | 100 Luciferase Assays |
| Quantity | 100 Reaction Kit |
| Content And Storage | Sufficient For: 100 luciferase assays in microplate wells • Gaussia Flash Assay Buffer, 5 mL (store at 4°C) • 100X Coelenterazine, 50 μL (store at -80°C) • Luciferase Cell Lysis Buffer (2X), 6 mL (store at room temperature) Kit may be stored at -80°C, or individually at indicated temperatures. |
| Assay | Reporter Enzyme, Luciferase Reporter Assay |
| Compatible Cells | Mammalian Cells |
| Detection Method | Bioluminescence |
| For Use With (Equipment) | Luminometer |
| Format | 96-well Plate, 384-well Plate |
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Safety and Handling
- Pierce Gaussia Luciferase Flash Assay Kit
- Danger
- Acute toxicity Category 3
- Flammable liquid Category 2
- Specific target organ toxicity after single exposure Category 1
- H225-Highly flammable liquid and vapour.
- H301-Toxic if swallowed.
- H311-Toxic in contact with skin.
- H331-Toxic if inhaled.
- H370-Causes damage to organs.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P403+P235-Store in a well-ventilated place. Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contain: methanol
For Research Use Only. Not for use in diagnostic procedures.
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