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Thermo Scientific™ Pierce™ Gentle Ag/Ab Elution Buffer, pH 6.6
Ensure high-yield, nondenaturing antibody purification using IgG affinity purification supports with these gentle buffer solutions.
160.00 € - 465.00 €
Specifications
Product Type | Elution Buffer |
---|---|
Product Line | Pierce™ |
Reagent Type | Elution Buffer |
pH | 6.6 |
Description | Pierce Gentle Ag/Ab Elution Buffer, pH 6.6 |
Produktcode | Merk | Description | Quantity | Prijs | Hoeveelheid en beschikbaarheid | ||||
---|---|---|---|---|---|---|---|---|---|
Produktcode | Merk | Description | Quantity | Prijs | Hoeveelheid en beschikbaarheid | ||||
10290544
|
Thermo Scientific™
21027 |
Pierce Gentle Ag/Ab Elution Buffer, pH 6.6 | 500 mL |
160.00 €
500mL |
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10017053
|
Thermo Scientific™
21013 |
Pierce Gentle Ag/Ab Elution Buffer, pH 6.6 | 3.75 L |
465.00 €
3.75 L |
Please sign in to purchase this item. Need a web account? Register with us today! | ||||
Beschrijving
Thermo Scientific™ Pierce IgG Binding and Elution Buffers ensure high-yield, nondenaturing antibody purification with IgG affinity purification supports, such as Protein A and Protein G agarose beads.
These products include IgG Binding Buffers specific for each immunoglobulin-binding protein (Protein A, Protein G, Protein A/G). The Pierce IgG Elution Buffer provides efficient dissociation and elution of antibodies from all three IgG-binding proteins, including Protein L. This low-pH elution buffer is also effective for most antibody-antigen or protein-protein affinity purification systems.The Gentle Ag/Ab Binding and Elution Buffer system uses near-neutral, high-salt conditions for elution, enabling even sensitive and labile antibodies (or other proteins) to be eluted from an affinity system without denaturation and inactivation. The phosphate-free binding buffer in this system is a necessary substitute for the Protein A binding buffer (pH 8), which precipitates with the Gentle Ag/Ab Elution Buffer.
Finally, the Mouse IgG1 Mild Elution Buffer, when used in combination with Protein A resin, enables selective elution and separation of the IgG1 subclass from total bound mouse IgG.
Highlights:
Three optimized binding buffers – for Protein A, Protein G or Protein A/G
Two elution buffer options – acidic (pH 2.8) or high salt (near-neutral)
Special system for mouse IgG1 – selective, two-stage elution from Protein A agarose
Item-Specific Features:
Protein A IgG Binding Buffer: pH 8.0; phosphate-based; contains glycerol and EDTA
Protein A/G IgG Binding Buffer: pH 8.0; phosphate-based; contains EDTA
Protein G IgG Binding Buffer: pH 5.0; contains 0.02% sodium azide
IgG Elution Buffer: pH 2.8; amine-based
Gentle Ag/Ab Binding Buffer: pH 8.0; phosphate-free; contains 0.05% sodium azide
Gentle Ag/Ab Elution Buffer: pH 6.6; high salt (ionic strength)
Mouse IgG1 Mild Elution Buffer: pH 6.0; contains 0.02% sodium azide
Includes:
Kits contain both binding and elution buffers
Specificatie
Elution Buffer | |
Elution Buffer | |
Pierce Gentle Ag/Ab Elution Buffer, pH 6.6 | |
Liquid |
Pierce™ | |
6.6 | |
4°C |
For Research Use Only. Not for use in diagnostic procedures.